Changes in Levels of Seminal Nitric Oxide Synthase, Macrophage Migration Inhibitory Factor, Sperm DNA Integrity and Caspase-3 in Fertile Men after Scrotal Heat Stress.

Background: This study observes changes in levels of seminal nitric oxide (NO), nitric oxide synthase (NOS), macrophage migration inhibitory factor (MIF), sperm DNA integrity, chromatin condensation and Caspase-3in adult healthy men after scrotal heat stress (SHS).

Methods: Exposure of the scrotum of 25 healthy male volunteers locally at 40-43°C SHS belt warming 40 min each day for successive 2 d per week. The course of SHS was continuously 3 months.

Scrotal heat stress causes sperm chromatin damage and cysteinyl aspartate-spicific proteinases 3 changes in fertile men.

Purpose: To observe changes in semen parameters, sperm DNA integrity, chromatin condensation and cysteinyl aspartate-spicific proteinases (Caspase-3) in adult healthy men after scrotal heat stress (SHS).

Methods: The scrotums of 19 healthy male volunteers were exposed to the condition of 40-43 °C SHS belt warming 40 min each day for successive 2 days per week. The course of SHS was continuously 3 months.

A new experimental three-dimensional, reticular intrauterine device (3-DRIUD) composed of nitinol and silicone rubber.

Background: The aim of this study was to explore a new three-dimensional, reticular intrauterine device (3-DRIUD) composed of nitinol and silicone rubber and to observe the contraceptive effect of the device in rats.

Study Design: Two contraceptive experiments were performed. In the first, female rats underwent bilateral placement of a 20.

[Breakpoints located by sequence tagged sites of AZFc microdeletion in Chinese Han population].

Objective: To investigate the breakpoints of the azoospermia factor c (AZFc) microdeletion in Chinese Han population.

Methods: We detected 9 sequence tagged sites (sY84, sY86, sY127, sY134, sY152, sY145, sY255, sY254 and sY157) to confirm AZFc microdeletions in the Y chromosome for patients with severe oligozoospermia or non-obstructive azoospermia by multiplex polymerase reaction. To locate the breakpoints of AZFc microdeletions, we analyzed 192 patients with sY255, sY254 and sY157 dele- ted by detecting sY1191, sY1197, sY1054, sY1125 and sY1206, respectively.

Quality of sperm obtained by penile vibratory stimulation and percutaneous vasal sperm aspiration in men with spinal cord injury.

The aim of this study was to explore sperm chromosomal aneuploidy and DNA integrity in infertile patients with spinal cord injury (SCI). Semen samples were collected from 12 infertile men with SCI by percutaneous vasal sperm aspiration (PVSA) and from 14 male SCI patients by penile vibratory stimulation (PVS). These semen samples as well as samples from 16 donors were analyzed using the hypo-osmotic swelling (HOS) test, the sperm chromatin dispersion test, terminal deoxynucleotidyl transferase-mediated terminal uridine nick-end labeling assay, and multicolor fluorescence in situ hybridization with probes specific for the chromosomes 13, 18, 21, X, and Y.

Sperm chromosomal aneuploidy and DNA integrity of infertile men with anejaculation.

Purpose: To explore sperm chromosomal aneuploidy, sperm membrane and DNA integrity in infertile patients with anejaculation.

Methods: Semen samples were collected from 18 infertile men with spinal cord injury (SCI) by penile vibratory stimulation (PVS) and from 14 psychogenic anejaculation (PA) patients by percutaneous vasal sperm aspiration (PVSA). These semen samples as well as samples from 16 donors were analyzed using the hypo-osmotic swelling (HOS) test, the sperm chromatin dispersion (SCD) test and multi-color fluorescence in situ hybridization (FISH) with probes specific for chromosomes 13, 18, 21, X and Y.

Effects of the crude extract of Polygala tenuifolia Willd on human sperm in vitro.

The aim of the present study is to analyze sperm membrane changes and the spermicidal effect in treatment with the crude extract from Polygala tenuifolia Willd (PTW) in vitro. The root of PTW was extracted in distilled water. Normal human spermatozoa were used to assess the spermicidal activity (Sander-Cramer assay) of the extract from the PTW root.

[Fast segmentation algorithm of high resolution remote sensing image based on multiscale mean shift].

Mean Shift algorithm is a robust approach toward feature space analysis and it has been used wildly for natural scene image and medical image segmentation. However, high computational complexity of the algorithm has constrained its application in remote sensing images with massive information. A fast image segmentation algorithm is presented by extending traditional mean shift method to wavelet domain.

Reversible contraceptive effect of the oviduct plug with nickel-titanium shape memory alloy and silicone rubber in rabbits.

Background: The aim of this study was to observe the contraceptive effectiveness and reversibility of the oviduct plug with nickel-titanium (Ni-Ti) shape memory alloy and silicone rubber in rabbits.

Study Design: The oviduct plugs with Ni-Ti shape memory alloy and silicone rubber were made as contraceptive devices. The frame of the oviduct plug is made of silicone rubber and Ni-Ti shape memory alloy wire.

[Mechanism for sperm immobilization activity of extract from Platycodon grandiflorum in vitro].

Objective: To explore the mechanism of spermicidal effect of crude extract and platycodin-D from Platycodon grandiflorum (PG) root in vitro.

Methods: Between February 2006 and December 2009, 38 fertile and healthy adult males were selected as donors. PG root was extracted and platycodin-D purified.

[Measurement of sperm DNA integrity by sperm chromatin dispersion test and TdT-mediated dUTP nick end labeling assay].

Objective: To compare the effects of sperm chromatin dispersion (SCD) test and TdT-mediated dUTP nick end labeling (TUNEL) assay in assessing the DNA fragmentation in human sperm.

Methods: Motile sperms were isolated from the semen samples obtained from 20 healthy fertile men and 32 clinically infertile patients by swim-up technique, and underwent SCD and TUNEL to analyze the DNA fragmentation.

Results: The rate of sperm with DNA damage of the infertile patients was 12.

Percutaneous vasal sperm aspiration and intrauterine insemination for infertile males with anejaculation.

Objective: To explore the effectiveness of percutaneous vasal sperm aspiration (PVSA) in combination with intrauterine insemination (IUI) to treat infertile men with anejaculation.

Design: Clinical study.

Setting: Department of reproductive endocrinology and andrology of a family planning research clinic.