Effects of Diet and Genetics on Growth Performance of Pigs in Response to Repeated Exposure to Heat Stress.

Heat stress (HS) is one of the costliest issues in the U.S. pork industry.

Iodine values of adipose tissue varied among breeds of pigs and were correlated with pork quality.

Our objectives were to investigate fatty acid composition variation amongst adipose tissue sites, breed effects on fat quality, and the relationship of pork fat quality to fresh pork quality. Barrows and gilts (n = 347) of five purebred and one commercial crossbred line were fed commercial swine diets with DDGS inclusion at 30% (as fed) from 31.8 kg body weight until 30-d prior to harvest at 111.

A novel and robust method for testing bimodality and characterizing porcine adipocytes of adipose tissue of 5 purebred lines of pig.

Adipocyte sizes from adipose tissue of mature animals form a bimodal distribution, thus reporting mean cell size is misleading. The objectives of this study were to develop a robust method for testing bimodality of porcine adipocytes, describe the size distribution with an informative metric, and statistically test hypertrophy and appearance of new small adipocytes, possibly resulting from hyperplasia or lipid filling of previously divided fibroblastic cells. Ninety-three percent of adipose samples measured were bimodal (P < 0.

Differential surface deposition of complement proteins on logarithmic and stationary phase Leishmania chagasi promastigotes.

Previous works demonstrated that various species of Leishmania promastigotes exhibit differential sensitivity to complement-mediated lysis (CML) during development. Upon exposure to normal human serum (NHS), cultures of Leishmania chagasi promastigotes recently isolated from infected hamsters (fewer than 5 in vitro passages) are CML-sensitive when in the logarithmic growth phase but become CML-resistant upon transition to the stationary culture phase. Visualization by light and electron microscopy revealed dramatic morphological differences between promastigotes from the 2 culture phases following exposure to NHS.

Population changes in Leishmania chagasi promastigote developmental stages due to serial passage.

Leishmania chagasi causes visceral leishmaniasis, a potentially fatal disease of humans. Within the sand fly vector, L. chagasi replicates as promastigotes which undergo complex changes in morphology as they progress from early stage procyclic promastigotes, to intermediate stage leptomonad and nectomonad promastigotes, and ultimately to terminal stage metacyclic promastigotes that are highly infective to vertebrates.

Reduced hamster usage and stress in propagating Leishmania chagasi promastigotes using cryopreservation and saphenous vein inoculation.

Leishmania chagasi, a causal agent of visceral leishmaniasis, requires passage through lab animals such as hamsters to maintain its virulence. Hamster infection is typically accomplished via cardiac puncture or intraperitoneal injection, procedures accompanied by risks of increased animal stress and death. The use of the hamster model also necessitates a regular supply of infected animals, because L.

Characterization of DNA sequences that confer complement resistance in Leishmania chagasi.

Serial passage of axenically cultured Leishmania chagasi promastigotes results in a progressive diminution in resistance to complement-mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low-passage cultures at stationary growth phase. As we previously reported, in a screen seeking to identify novel genes involved in CML resistance: (1) a genomic cosmid library derived from DNA of CML-resistant L. chagasi promastigotes was transfected into high-passage (constitutively CML-sensitive) L.

Continuous-wave rotational Raman laser in H(2).

A diode-pumped, far-off-resonance cw Raman laser in H(2) with rotational Stokes emission is reported for the first time to our knowledge. The Raman laser can produce single-wavelength emission at either 830 nm (rotational Stokes) or 1180 nm (vibrational Stokes) depending on the frequency tuning of the pump laser. The mirrors for the rotational cw Raman laser are easier to produce; the laser also exhibits a wider continuous tuning range and is less sensitive to thermal effects than the previously studied vibrational Raman laser [Opt.

Tissue specificity of a baculovirus-expressed, basement membrane-degrading protease in larvae of Heliothis virescens.

ScathL is a cathepsin L-like cysteine protease from the flesh fly, Sarcophaga peregrina, which digests components of the basement membrane during insect metamorphosis. A recombinant baculovirus (AcMLF9.ScathL) expressing ScathL kills larvae of the tobacco budworm Heliothis virescens significantly faster than the wild type virus and triggers melanization and tissue fragmentation shortly before death.

High power 7-GHz bandwidth external-cavity diode laser array and its use in optically pumping singlet delta oxygen.

Using a 14-mm thick volume Bragg grating, spectral bandwidth of a cw-operated diode laser array is narrowed to 7 GHz (FWHM). Total output power reaches 13.5 W cw, of which 86% is in the 7-GHz band.

Damage threshold and surface distortion measurement for high-reflectance, low-loss mirrors to 100+ MW/cm(2) cw laser intensity.

By frequency-stabilizing the output from an Erbium fiber amplifier at 1580 nm to a high-finesse cavity (finesse ~6300) formed by two high-reflectance, low-loss, concave mirrors, we achieve 22.4+/-2.0 kW intracavity circulating power and 101+/-9 MW/cm(2) cw intracavity intensities on the surfaces of the mirrors.

Doppler-induced unidirectional operation of a continuous-wave Raman ring laser in H2.

We demonstrate stable operation of a diode-pumped cw Raman ring laser in diatomic hydrogen gas. Doppler-induced asymmetry between the the forward and the backward Raman gains leads to inherent unidirectional operation in the forward direction without intracavity optical elements. Use of the ring-cavity geometry dilutes the deleterious effects of thermal lensing and significantly reduces optical feedback to the pump laser.