HSV infected RAJI-cells specify HSV specific immediate early and/or early DNA-binding proteins.

Herpes Simplex Virus specified DNA-binding proteins were purified from virus infected VERO and RAJI cells. The expression of the proteins differed depending on the type of the host cell. Polypeptides corresponding to HSV-1 specific ICP 8 and HSV-2 specific ICSP 11/12 were the major products of HSV-infected VERO cells.

Serum C-reactive protein determination in acute pelvic inflammatory disease.

We have studied the role of serum C-reactive protein determination in the diagnosis of acute pelvic inflammatory disease. Acute-phase serum C-reactive protein concentration reflected the extent and the severity of pelvic inflammatory disease more closely than erythrocyte sedimentation rate or white blood cell count determinations. We recommend that both C-reactive protein concentration and erythrocyte sedimentation rate should be routinely used to augment the clinical diagnosis of pelvic inflammatory disease.

Anatomic sites of upper genital tract infection.

The microbiologic correlates of upper genital tract infection were studied among 36 women with suspected upper genital tract infection and 11 control women undergoing tubal ligation. Laparoscopic evidence of confirmed salpingitis was seen in 25 (69%) of the 36 women. Pathologic evidence of endometritis was present in 26 women (72%), and 22 (85%) of them had salpingitis as well.

Comparison of central nervous system actions of taurine and N-pivaloyltaurine.

N-Acetyl, N-propionyl, and N-pivaloyl derivatives of taurine were synthesized by applying a modified Schotten-Bauman method starting from taurine and using the corresponding acid chloride or acid anhydride for direct acylation reactions. The central nervous system actions of these lipid soluble taurine derivatives, which were presumed to pass the blood-brain barrier, were studied and compared to those of taurine in mice. A large dose (15 mmol/kg) of intraperitoneally administered taurine lengthened the pentobarbitone induced sleep by 30%.

Chlamydial endometritis.

Endometrial biopsies were obtained from 32 women with suspected pelvic inflammatory disease, of whom 23 (72%) had histopathological evidence of endometritis. Chlamydia trachomatis was isolated from the endometria of nine (39%) women (chlamydia group) but not from the other 14 (non-chlamydia group). Severe plasma cell endometritis and lymphoid follicles with transformed lymphocytes were significantly more common in the chlamydia group than in the non-chlamydia group.

Serum antibodies to the major HSV-2-specified DNA-binding protein in patients with an acute HSV infection or cervical neoplasia.

The major HSV-2-specified DNA-binding protein (ICSP 11/12) was purified from HSV-2-infected cells. ELISA and immunoblotting techniques were used to study its antigenicity in HSV-infected patients and patients with cervical neoplasia and control women. Patients with an acute HSV-2 infection had clearcut antibody responses to the purified ICSP 11/12 preparation.

Detection of herpes simplex virus in women with acute pelvic inflammatory disease.

Four women are described with acute salpingitis confirmed by laparoscopy who had herpes simplex virus (HSV) isolated from the cervix or the upper genital tract (endometrium, fallopian tube, or cul-de-sac) or both. None of the patients had overt genital herpes, but one had typical HSV cervitis on a cervicovaginal smear stained with Papanicolaou's stain, one had a significant change in level of antibodies to HSV, and one had an endometrial biopsy specimen positive for HSV antigen. There are at least three potential explanations for these findings: chronic viral shedding, viral reactivation caused by acute pelvic inflammatory disease (PID), or that the PID was actually caused by HSV.

Haemophilus influenzae causes purulent salpingitis.

We describe two patients with laparoscopically diagnosed, severe acute salpingitis who had nontypable Haemophilus influenzae isolated directly from the fallopian tubes. Nontypable H. influenzae should be recognized as an important genital pathogen.

Endometritis and acute salpingitis associated with Chlamydia trachomatis and herpes simplex virus type two.

A patient who had endometritis confirmed by endometrial biopsy and acute salpingitis confirmed by laparoscopy is reported. Chlamydia trachomatis and herpes simplex virus type two were isolated from the endometrial cavity and the fallopian tube. The histopathologic findings of the endometritis were similar to those frequently seen in chronic chlamydial eye disease or chlamydial cervicitis.

Immunoblotting and enzyme-linked immunosorbent assay analysis of serological responses in patients infected with herpes simplex virus types 1 and 2.

Serological responses to soluble membrane and cytoplasmic antigens specified by herpes simplex type 1 (HSV1) and 2 (HSV2) were studied by immunoblotting and by enzyme-linked immunosorbent assay (ELISA). In the immunoblotting test, polypeptides migrating like the HSV1-specified glycoprotein C showed type-specific reactivity but could not always be detected. The immunoblotting and ELISA results were in agreement when antibody responses to HSV1- and HSV2-specified antigens were compared, and they allowed the identification of patients with HSV2 and/or HSV1 antibodies.

Immunoblotting and ELISA analysis of HSV-1 and HSV-2-specified polypeptides by using an immunoblocking method.

Herpes Simplex Virus (HSV) specified polypeptide antigens were analyzed by immunoblotting and enzyme-linked immunosorbent assay (ELISA) methods. In general, the virus specified proteins showed marked cross-reactivity. However, the HSV-1 specified glycoprotein C could be detected by HSV-1 infected rabbit antiserum only.

Purification of herpes simplex virus-specified major DNA-binding proteins and detection of antibodies to them in human and rabbit sera.

We purified herpes simplex virus type 1 and 2-specified nonstructural DNA-binding proteins (ICP8 and ICSP 11/12, respectively) from infected Vero cells and applied them in ELISA test to analyse human and rabbit sera. Only a weak immune response to the ICP8 and ICSP 11/12 could be demonstrated following experimental HSV-1 or HSV-2 infections of rabbits. Low levels of ICSP8 antibodies were found also in acute HSV infections of man.

Quality control of insulin radioreceptor assay for human erythrocytes. Effect of ageing of mono-125I-Tyr-A14-insulin preparation.

The quality control of insulin radioreceptor assay for human erythrocytes is based on the storage of erythrocyte preparations in Hepes buffer of pH 8.0, containing 10 g/l of albumin and 20 mmol/l of glucose. The change of erythrocytes into spherocytes and crenated cells reduces the apparent number of insulin receptors in a relatively constant way by less than 8% a week after 10 days of storage.

Enrichment of early HSV-induced proteins in phosphonoformate-treated cells.

The early HSV-specified proteins were selectively enriched in phosphonoformate (PFA)-treated Vero cells, i.e. in the absence of virus DNA synthesis.

HLA antigens in rubella seronegative young adults.

HLA antigen frequencies, including those of five HLA-D specificities, were studied in 91 rubella seronegative subjects who were found in screening programs for rubella vaccination. No significant differences in antigen frequencies between rubella seronegative subjects and the reference population could be found.

Drugs for Parkinson's disease reduce tremor induced by physostigmine.

The effects of anticholinergic and dopaminergic drugs used for Parkinson's disease were studied on the tremor induced by physostigmine (0.3-3.0 mg/kg) in rats.

Fibrinogen: a comparison of an immunoturbidimetric method with four conventional methods.

A rapid and simple immunochemical method for fibrinogen determination is described. The method is based on measuring the turbidity caused by antigen-antibody complexes with an enzyme analyser using diluted antiserum as a starting reagent. This immunoturbidimetric method correlates well with the immunodiffusion method and with two of the thrombin methods.

Vaccination of seronegative high-risk populations with attenuated rubella vaccine.

A total of 590 young females were screened for rubella antibodies. About 10% were seronegative, the frequency being lowest in vaccinated school girls. No coincidence between rubella and mumps seronegativity was observed.

The Finnish leukaemia group: levamisole in maintenance therapy of acute myeloid leukemia in adults.

The Finnish Leukaemia Group has carried out a randomized, multicenter trial to study the effect of levamisole on the remission maintained with 6-mercaptopurine and methotrexate in acute myeloid leukaemia in adults. Levamisole was given on 3 consecutive days every 2 weeks. Twenty-five patients received only chemotherapy, while 26 patients received levamisole as well.

Quantification of tremor in rats induced by physostigmine.

A simple and accurate device for recording tremor intensity in unanaesthetized and unrestrained rats is described. The physical measures of tremor are shown to have several advantages over previous devices. First, the new apparatus, unlike some earlier ones, does not restrict the animal's movements to an unusually small cage, with weighty mechanical devices or with electrical leads.

Macroglobulinemia with abdominal symptoms caused by intestinal extracellular macroglobulin.

A 54-year-old woman had a marked splenomegaly, diarrhoea and abdominal cramps. Her serum contained monoclonal IgM with lambda light chains, and lambda light chains were also excreted in the urine. Bone marrow and spleen punctures failed to reveal the classic morphological changes associated with Waldenström's macroglobulinemia.