Decision Considerations and Strategies for Lip Surgery in Patients with Cleft lip/Palate: A Qualitative Study.

Objective: To qualitatively assess surgeons' decision making for lip surgery in patients with cleft lip/palate (CL/P).

Design: Prospective, non-randomized, clinical trial.

Setting: Clinical data institutional laboratory setting.

Decision Considerations and Strategies for Lip Surgery in Patients with Cleft lip/Palate: A Qualitative Study.

Objective: To qualitatively assess surgeons decision making for lip surgery in patients with cleft lip/palate (CL/P).

Design: Prospective, non-randomized, clinical trial.

Setting: Clinical data institutional laboratory setting.

Automating Anesthesiology Resident Case Logs Reduces Reporting Variability.

Background: The Accreditation Council for Graduate Medical Education (ACGME) case log system for anesthesiology resident training relies on subjective categorization of surgical procedures and lacks clear guidelines for assigning credit roles. Therefore, resident reporting practices likely vary within and between institutions. Our primary aim was to develop a systematic process for generating automated case logs using data elements extracted from the electronic health care record.

Curriculum Vitae Workshops: Promoting Transparency in Alpha Omega Alpha While Serving Student Needs.

Objective: To promote equity in the Alpha Omega Alpha Honor Medical Society (AOA) selection process, clear and timely communication of eligibility criteria is needed. Herein, the authors describe and assess the effectiveness of a novel method for improving transparency in the AOA selection process while also teaching students key professional development skills.

Methods: The authors hosted curriculum vitae (CV) workshops for interested medical students.

Management of submucous cleft palate.

Purpose Of Review: Submucous cleft palate (SMCP) represents an uncommon congenital palatal anomaly with a variable rate of velopharyngeal dysfunction or resulting speech abnormality. Classic teaching regarding management of this entity involves delayed repair until a perceptual speech assessment by a skilled speech-language pathologist can be performed, typically at age 3-5 years. An assessment of timing of intervention, surgical techniques, and patient comorbidities is critical for optimized outcomes.

Regulation of the formation and trafficking of vesicles from Golgi by PCH family proteins during chemotaxis.

Previous study demonstrated that WASP localizes on vesicles during Dictyostelium chemotaxis and these vesicles appear to be preferentially distributed at the leading and trailing edge of migrating cells. In this study, we have examined the role of PCH family proteins, Nwk/Bzz1p-like protein (NLP) and Syndapin-like protein (SLP), in the regulation of the formation and trafficking of WASP-vesicles during chemotaxis. NLP and SLP appear to be functionally redundant and deletion of both nlp and slp genes causes the loss of polarized F-actin organization and significant defects in chemotaxis.

Role of RacC for the regulation of WASP and phosphatidylinositol 3-kinase during chemotaxis of Dictyostelium.

WASP family proteins are key players for connecting multiple signaling pathways to F-actin polymerization. To dissect the highly integrated signaling pathways controlling WASP activity, we identified a Rac protein that binds to the GTPase binding domain of WASP. Using two-hybrid and FRET-based functional assays, we identified RacC as a major regulator of WASP.

WASP-interacting protein is important for actin filament elongation and prompt pseudopod formation in response to a dynamic chemoattractant gradient.

The role of WASP-interacting protein (WIP) in the process of F-actin assembly during chemotaxis of Dictyostelium was examined. Mutations of the WH1 domain of WASP led to a reduction in binding to WIPa, a newly identified homolog of mammalian WIP, a reduction of F-actin polymerization at the leading edge, and a reduction in chemotactic efficiency. WIPa localizes to sites of new pseudopod protrusion and colocalizes with WASP at the leading edge.

Sucrase-isomaltase ontogeny: synergism between glucocorticoids and thyroxine reflects increased mRNA and no change in cell migration.

During postnatal maturation of the rat small intestine, glucocorticoid hormones (GC) and thyroxine (T4) act synergistically to elicit a precocious increase of sucrase activity. The current work shows that the synergistic effect on sucrase activity is paralleled by increased steady-state levels of sucrase-isomaltase mRNA. The enhancing effects of T4 on dexamethasone (DEX)-induced sucrase activity was seen even after prolonged treatment (9 days).

Intestinal maturation in mice lacking CCAAT/enhancer-binding protein alpha (C/EPBalpha).

In rodents, there is a surge of intestinal expression of CCAAT/enhancer-binding protein alpha (C/EBPalpha) in the late fetal phase just before morphological maturation and the onset of expression of numerous epithelial genes. To investigate directly the hypothesis that C/EBPalpha plays a causal role in the latter phenomena, we have assessed both structural and functional maturation in neonatal intestine from C/EBPalpha-null mice and their littermates. No effects of C/EBPalpha genotype were observed on mucosal architecture or on the size of the proliferative zone in the intestinal crypts.

Speech fluency: effect of age, gender and context.

The effects of age, gender and task on speech fluency performance in three speaking conditions were examined in 78 older (55-92 years) talkers. Results were analyzed for significant differences (p < or = 0.01) as a function of age, gender and task between speakers groups formed on increasingly older chronological age and as compared to a young (25-35 years) control group.

The sucrase-isomaltase structural gene (Si-s) and a regulatory gene (Si-r) are closely linked to esterase-26 (Es-26) on mouse chromosome 3.

A cDNA clone of the rat sucrase-isomaltase (SI) structural gene detected two distinct patterns of DNA fragments on Southern blots of mouse DNA. Screening of 18 AKXL and 25 AKXD recombinant inbred (RI) strains revealed that all bands in each pattern co-segregated and there were no (0/43) recombinants with Es-26 on mouse Chromosome (Chr) 3. Since CBA/CaJ mice have approximately threefold less sucrase activity than other strains, we intercrossed them with SJL/J mice to map the previously identified SI regulatory gene, Si-r.

Hormonal regulation of the mRNA for cysteine-rich intestinal protein in rat jejunum during maturation.

Cysteine-rich intestinal protein (CRIP) has been implicated as an important zinc-binding protein in the rat intestine. However, its specific role remains undefined. As an approach to the ultimate elucidation of the function of CRIP, we have explored the role of glucocorticoids and L-thyroxine (T4) in the increase of CRIP mRNA that occurs during postnatal development.

Development and tissue distribution of sucrase-isomaltase mRNA in rats.

Previous studies of sucrase-isomaltase (SI) activities have shown this complex to be absent in the suckling rat and to appear during the weaning period. We describe here the cloning of a heterologous SI cDNA and its use for the quantitation of SI mRNA as a first step toward understanding the molecular basis of SI development. A survey of RNA from 12 tissues of mature rats by Northern blot analysis showed a 6-kb band of SI mRNA only in the small intestine.

Kinetics of circulating corticosterone in infant rats.

Corticosterone plays an important role in the regulation of postnatal development in the rat. Basal concentrations of plasma corticosterone increase markedly during the 3rd wk of life. To date, however, the physiologic bases of this increase have remained unclear.

Effects of soy formulas on mineral metabolism in term infants.

We studied 40 healthy term infants who received a soy-based formula containing either a single carbohydrate (glucose polymers) or dual carbohydrates (glucose polymers and sucrose). Ten exclusively breast-fed infants served as controls for the first four months of the study. All infants were studied at 2 weeks, 2 months, and 4 months of age for anthropometric development, biochemical values, and bone mineral content.

Circulating corticosterone in the infant rat: the mechanism of age and thyroxine effects.

Infant rats exhibit a marked rise in serum concentrations of corticosterone between postnatal days 12 and 24. As this rise appears to be cued by L-thyroxine, the aim of the current study was to determine the physiological bases of the effects of both age and thyroid status on serum corticosterone. The in vivo response to either adrenocorticotropic hormone (60 mU/g body weight) or dibutyryl 3',5'-cyclic adenylate (0.

Duodenal uptake of lead by suckling and weanling rats.

The aim of the current study was to determine the site of absorption of Pb by suckling rats. When 203PbCl2 (carrier-free) plus Pb-acetate (50 micrograms/g BW) was intragastrically administered to rat pups aged 10 and 14 days, mucosal uptake of Pb, measured 2 h after intubation, was manyfold greater in the duodenum than in other regions of the small intestine. By 24 days of age, this duodenal uptake was no longer apparent.

Effect of cortisone on intestinal uptake of lead in the suckling rat.

A previous study showed that there is extensive uptake of lead by the duodenum and the ileum of the suckling rat. At both sites, uptake declines with advancing age. This paper reports the effects of administration of cortisone acetate on in vivo lead uptake in 14- to 15-day-old rats.

Hormonal control of postnatal development of ileal neuraminidase and acid beta-galactosidase.

This study examines the effect of changes of thyroid and glucocorticoid status on the development of ileal neuraminidase and acid beta-galactosidase. Thyroxine (T4) administration on postnatal days 6-13 had no effect on the activity of either enzyme. In contrast, a single injection of cortisone acetate on day 6 caused a precocious reduction of the activities of both enzymes.

Intestinal maturation: in vivo magnesium transport.

We investigated with an in vivo single pass perfusion technique net transport of magnesium in the jejunum, ileum and colon segments of suckling, weanling and adolescent rats. The concentration of magnesium in the isotonic sodium chloride solution perfused through the intestinal segments was either below (0 and 0.5 mmoles/liter) or above (1, 2.