Effector proteins of Funneliformis mosseae BR221: unravelling plant-fungal interactions through reference-based transcriptome analysis, in vitro validation, and protein‒protein docking studies.

Background: Arbuscular mycorrhizal (AM) fungi form a highly adaptable and versatile group of fungi found in natural and man-managed ecosystems. Effector secreted by AM fungi influence symbiotic relationship by modifying host cells, suppressing host defense and promoting infection to derive nutrients from the host. Here, we conducted a reference-based transcriptome sequencing of Funneliformis mosseae BR221 to enhance understanding on the molecular machinery involved in the establishment of interaction between host and AM fungi.

Recreating in vitro tripartite mycorrhizal associations through functional bacterial biofilms.

Arbuscular mycorrhizal fungi (AMF) and beneficial bacteria are found naturally associated with most terrestrial plant roots. While it is now well known that bacteria colonize AMF and can form aggregates and biofilms, little is known about how interactions between bacterial communities and AMF take place under both in situ and in vitro conditions. We investigated the impact of inoculation with AMF-associated bacteria (AABs) of AMF by in vitro recreation of the interaction on synthetic growth media in a two-compartment Petri plate system.

Diversity and Functionalities of Unknown Mycorrhizal Fungal Microbiota.

Beneficial ecosystem services provided by arbuscular mycorrhizal fungi (AMF) are the outcome of their synergistic actions with diverse bacterial communities (AMF-associated bacteria; AAB) living in strict association with AMF hyphae and spores. Herein, bacterial diversity associated with 6 AMF species from 33 different co-cultures belonging to order Glomerales and Diversisporales were identified, using a combination of culture-dependent functional analyses and amplicon sequencing. Overall, 231 bacterial strains were isolated from the AMF spores and hyphae which covered 30 bacterial genera and 52 species.

Review of patents for agricultural use of arbuscular mycorrhizal fungi.

Mycorrhizal biotechnology has emerged as a major component of sustainable agriculture and allied activities. Innovations related to its role in agriculture, land reclamation, forestry, and landscaping are well recognized. This review presents the evolution of innovations worldwide related to arbuscular mycorrhizal fungi (AMF) in the past two decades, from 2000 to April 2020, and maintains that such innovations must continue in the future.