Biological metal organic framework (bio-MOF) of glucoamylase with enhanced stability.

The handling of conventional enzyme- metal organic framework (MOF) composites is big challenge due to their nano-sized and lightweight structure with low density. Also, conventional MOFs are derived from non-renewable petroleum feedstock which makes them inherent toxic and non-biodegradable. To overcome these difficulties, recently, green, renewable framework material composite, biological metal-organic frameworks (bio-MOFs) have intrigued as a novel class of porous materials.

Entrapment of surfactant modified lipase within zeolitic imidazolate framework (ZIF)-8.

Mostly, enzyme activity is reduced after immobilization of enzyme within MOF due to unfavourable conformational changes occurred during the immobilization procedure. In this context, lipase was activated by surfactants (in order to get a highly active enzyme) followed by encapsulation within zeolitic imidazolate framework (ZIF)-8 via one-pot facile self-assembly method. The prepared lipase-SDS ZIF-8 exhibited 250% enhanced activity compared to native form.