Is calcium deficiency the real cause of bitter pit? A review.

Bitter pit is a disorder affecting the appearance of apples. Susceptibility is genetically controlled by both the cultivar and rootstock, with both environmental and horticultural factors affecting its severity and proportional incidence. Symptoms appear more frequently at the calyx end of the fruit and consist of circular necrotic spots, which take on a "corky" appearance visible through the peel.

Relating microtensiometer-based trunk water potential with sap flow, canopy temperature, and trunk and fruit diameter variations for irrigated 'Honeycrisp' apple.

Instrumentation plays a key role in modern horticulture. Thus, the microtensiomenter, a new plant-based sensor that continuously monitors trunk water potential (Ψ) can help in irrigation management decisions. To compare the response of the Ψ with other continuous tree water status indicators such as the sap flow rate, the difference between canopy and air temperatures, or the variations of the trunk and fruit diameter, all the sensors were installed in 2022 in a commercial orchard of 'Honeycrisp' apple trees with M.

Rootstock effects on leaf function and isotope composition in apple occurred on both scion grafted and ungrafted rootstocks under hydroponic conditions.

Rootstocks are used in modern apple production to increase productivity, abiotic and biotic stress tolerance, and fruit quality. While dwarfing for apple rootstocks has been well characterized, the physiological mechanisms controlling dwarfing have not. Previous research has reported rootstock effects on scion water relations.

Combining thermal imaging and soil water content sensors to assess tree water status in pear trees.

Volumetric soil water content is commonly used for irrigation management in fruit trees. By integrating direct information on tree water status into measurements of soil water content, we can improve detection of water stress and irrigation scheduling. Thermal-based indicators can be an alternative to traditional measurements of midday stem water potential and stomatal conductance for irrigation management of pear trees ( L.

Transcriptomic approach to uncover dynamic events in the development of mid-season sunburn in apple fruit.

Apples grown in high heat, high light, and low humidity environments are at risk for sun injury disorders like sunburn and associated crop losses. Understanding the physiological and molecular mechanisms underlying sunburn will support improvement of mitigation strategies and breeding for more resilient varieties. Numerous studies have highlighted key biochemical processes involved in sun injury, such as the phenylpropanoid and reactive oxygen species (ROS) pathways, demonstrating both enzyme activities and expression of related genes in response to sunburn conditions.

Apple rootstocks affect functional leaf traits with consequential effects on carbon isotope composition and vegetative vigour.

Composite trees combine optimal traits from both the rootstock and the scion. Dwarfing rootstocks are commonly used to reduce shoot vigour and improve fruit quality and productivity. Although growth habits of different rootstocks have been clearly described, the underlying physiological traits affecting scion vigour are not well understood.

Microtensiometers Accurately Measure Stem Water Potential in Woody Perennials.

Stem water potential (Ψ) is considered to be the standard measure of plant water status. However, it is measured with the pressure chamber (PC), an equipment that can neither provide continuous information nor be automated, limiting its use. Recent developments of microtensiometers (MT; FloraPulse sensors), which can continuously measure water tension in woody tissue of the trunk of the tree, can potentially highlight the dynamic nature of plant water relations.

Soil health indicators for Central Washington orchards.

Soil health assessment can be a critical soil testing tool that includes biological and physical indicators of soil function related to crop and environmental health. Soil health indicator minimum data sets should be regional and management goal specific. The objective of this study was to initiate the steps to develop a soil assessment tool for irrigated orchard soils in Central Washington, United States including defining objectives, gathering baseline data and selecting target indicators.

Plant Uptake of Lactate-Bound Metals: A Sustainable Alternative to Metal Chlorides.

Global agricultural intensification has prompted investigations into biostimulants to enhance plant nutrition and soil ecosystem processes. Metal lactates are an understudied class of organic micronutrient supplement that provide both a labile carbon source and mineral nutrition for plant and microbial growth. To gain a fundamental understanding of plant responses to metal lactates, we employed a series of sterile culture-vessel experiments to compare the uptake and toxicity of five metals (Zn, Mn, Cu, Ni, and Co) supplied in lactate and chloride salt form.

Photoselective Protective Netting Improves "Honeycrisp" Fruit Quality.

High temperatures, wind, and excessive sunlight can negatively impact yield and fruit quality in semi-arid apple production regions. Netting was originally designed for hail protection, but it can modify the light spectrum and affect fruit quality. Here, pearl, blue, and red photoselective netting (≈20% shading factor) was installed in 2015 over a commercial "Cameron Select Honeycrisp" orchard.

Water Deficit Timing Affects Physiological Drought Response, Fruit Size, and Bitter Pit Development for 'Honeycrisp' Apple.

Irrigation is critical to maintain plant growth and productivity in many apple-producing regions. 'Honeycrisp' apple characteristically develops large fruit that are also susceptible to bitter pit. Limiting fruit size by restricting irrigation may represent an opportunity to control bitter pit in 'Honeycrisp'.

Genotypic variation in nitrogen isotope discrimination in Populus balsamifera L. clones grown with either nitrate or ammonium.

Intraspecific variability in nitrogen use has not been comprehensively assessed in a natural poplar species. Here, a nitrogen isotope mass balance approach was used to assess variability in nitrogen uptake, assimilation and allocation traits in 25 genotypes from five climatically dispersed provenances of Populus balsamifera L. grown hydroponically with either nitrate or ammonium.

Non-destructive Measurement of Calcium and Potassium in Apple and Pear Using Handheld X-ray Fluorescence.

Calcium and potassium are essential for cell signaling, ion homeostasis and cell wall strength in plants. Unlike nutrients such as nitrogen and potassium, calcium is immobile in plants. Localized calcium deficiencies result in agricultural losses; particularly for fleshy horticultural crops in which elemental imbalances in fruit contribute to the development of physiological disorders such as bitter pit in apple and cork spot in pear.

Variation in fluxes estimated from nitrogen isotope discrimination corresponds with independent measures of nitrogen flux in Populus balsamifera L.

Acquisition of mineral nitrogen by roots from the surrounding environment is often not completely efficient, in which a variable amount of leakage (efflux) relative to gross uptake (influx) occurs. The efflux/influx ratio (E/I) is, therefore, inversely related to the efficiency of nutrient uptake at the root level. Time-integrated estimates of E/I and other nitrogen-use traits may be obtainable from variation in stable isotope ratios or through compartmental analysis of tracer efflux (CATE) using radioactive or stable isotopes.

Nitrogen isotope discrimination as an integrated measure of nitrogen fluxes, assimilation and allocation in plants.

Fractionation of nitrogen isotopes between a plant and its environment occurs during uptake and assimilation of inorganic nitrogen. Fractionation can also occur between roots and the shoot. Under controlled nitrogen conditions, whole-plant and organ-level nitrogen isotope discrimination (Δ(15) N) is suggested to primarily be a function of three factors: nitrogen efflux back to the substrate relative to gross influx at the root (efflux/influx), the proportion of net influx assimilated in the roots and the export of remaining inorganic nitrogen for assimilation in the leaves.

Quantifying remobilization of pre-existing nitrogen from cuttings to new growth of woody plants using 15N at natural abundance.

Background: For measurements of nitrogen isotope composition at natural abundance, carry-over of pre-existing nitrogen remobilized to new plant growth can cause deviation of measured isotope composition (δ15N) from the δ15Nof newly acquired nitrogen. To account for this problem, a two-step approach was proposed to quantify and correct for remobilized nitrogen from vegetative cuttings of Populus balsamifera L. grown with either nitrate (δ15N = 58.

Whole-plant and organ-level nitrogen isotope discrimination indicates modification of partitioning of assimilation, fluxes and allocation of nitrogen in knockout lines of Arabidopsis thaliana.

The nitrogen isotope composition (δ¹⁵N) of plants has potential to provide time-integrated information on nitrogen uptake, assimilation and allocation. Here, we take advantage of existing T-DNA and γ-ray mutant lines of Arabidopsis thaliana to modify whole-plant and organ-level nitrogen isotope composition. Nitrate reductase 2 (nia2), nitrate reductase 1 (nia1) and nitrate transporter (nrt2) mutant lines and the Col-0 wild type were grown hydroponically under steady-state NO₃⁻ conditions at either 100 or 1000 μM NO₃⁻ for 35 days.

Temperature-driven plasticity in growth cessation and dormancy development in deciduous woody plants: a working hypothesis suggesting how molecular and cellular function is affected by temperature during dormancy induction.

The role of temperature during dormancy development is being reconsidered as more research emerges demonstrating that temperature can significantly influence growth cessation and dormancy development in woody plants. However, there are seemingly contradictory responses to warm and low temperature in the literature. This research/review paper aims to address this contradiction.

Magnetic resonance microimaging indicates water diffusion correlates with dormancy induction in cultured hybrid poplar (Populus spp.) buds.

Water content and mobility, which are factors known to be associated with dormancy induction in woody plants on a tissue level, were measured using non-destructive magnetic resonance microimaging (MRMI). Two cultured poplar clones ('Okanese' - temperature-insensitive dormancy and 'Walker' - temperature-sensitive dormancy) were subjected to dormancy differentiating temperature regimes, 18.5/3.