Characterization of combustion-generated carbonaceous nanoparticles by size-dependent ultraviolet laser photoionization.

Photoelectric charging of particles is a powerful tool for online characterization of submicrometer aerosol particles. Indeed photoionization based techniques have high sensitivity and chemical selectivity. Moreover, they yield information on electronic properties of the material and are sensitive to the state of the surface.

On the characterization of nanoparticles emitted from combustion sources related to understanding their effects on health and climate.

This work describes the use of well-controlled laboratory flames to produce aerosols of organic carbon (OC) as model particles representative of the OC fraction of combustion-generated particulate matter emissions in fresh exhausts. Water-particle interactions are explored in two specific cases. In the first case, particles are exposed to saturated environments and come into direct contact with liquid water by bubbling flame samples through a column of water.

Interaction between combustion-generated organic nanoparticles and biological systems: in vitro study of cell toxicity and apoptosis in human keratinocytes.

The purpose of this work was to evaluate the effect of flame-generated nucleation mode particles with an organic carbon structure on growth and apoptosis in immortalized human keratinocytes. In this study, cells were stimulated with nanoparticles collected from flames that produce only nucleation mode particles operating with a fuel:air mixture typical of low-emission combustion systems. Cytotoxicity as a function of particle concentration was monitored by fluorescence-activated cell sorting (FACS) analysis, and apoptosis was observed by FACS using DNA fragmentation and hypodiploidism and confirmed by annexin assay.

Measurements of nanoparticles of organic carbon and soot in flames and vehicle exhausts.

We measured the size distribution and UV extinction spectra of carbonaceous nanoparticles present in the size range of 1-100 nm in the exhausts of 2004 model gasoline and diesel powered vehicles and compared the results with those obtained in premixed flames. In addition to soot particles, nanoparticles of organic carbon (NOC) were measured in the emissions of these test vehicles in significant number and mass concentrations. The number and mass concentration of NOC was higher than soot in gasoline vehicle emissions.