Human papillomavirus detection in cervical scrapes from women attended in the Family Health Program.

Objectives: to survey the prevalence of human papillomavirus, associated risk factors and genotype distribution in women who were referred to cervical cancer screening when attended in a Family Health Program.

Method: we conducted a cross-sectional survey, investigating 351 women. Polymerase chain reaction for DNA amplification and restriction fragment length polymorphism analysis were used to detect and typify the papillomavirus.

Prevalence of human papillomavirus and Epstein-Barr virus DNA in penile cancer cases from Brazil.

Penile cancer is a potentially mutilating disease. Although its occurrence is relatively rare worldwide, penile cancer rates can be high in developing countries. A few studies have been conducted on the involvement of human papillomavirus (HPV) in penile carcinoma, which have found HPV present in 30-70% of penile malignant lesions, with a higher prevalence of HPV 16 and 18.

Human papillomavirus detection and typification in cutaneous and mucosal lesions of HIV-seropositive patients.

High-risk human papillomavirus (HPV) in a lesion is related to an increased chance of neoplasic transformation, especially when with immunosuppression, as in HIV infection. We investigated HPV frequency in cutaneous and mucosal lesions of HIV-seropositive male patients. The frequency of malignancy, its association with the HPV type detected and some clinical variables were also assessed.

Bowenoid papulosis in a patient with AIDS treated with imiquimod: case report.

A 53-year-old male patient with acquired immunodeficiency syndrome (AIDS) was treated with topical immunomodulator imiquimod for bowenoid papular. Clinically the lesions presented as condilomatous and papulous changes with color varying from skin color to grayish. The lesions were located in the glans and in the dorsum of the penis.

HPV 16 detection in cervical lesions, physical state of viral DNA and changes in p53 gene.

Context: Persistent infection with high risk human papillomavirus (HPV) has been linked to cervical carcinoma. Integration of viral DNA into host cell DNA is essential for this cancer development, promoting disruption of the HPV E2 gene, thus leading to unregulated increases in E6 and E7 proteins and inactivating the products of p53 and Rb tumor suppressor genes.

Objective: To investigate HPV 16 infection in cervical lesions, physical state of viral DNA and p53 gene alterations in a group of women attending a public health service.

Analysis of the p53 gene and papillomavirus detection in smears from cervical lesions.

Context: Alterations of the p53 tumor suppressor gene are correlated with a critical step in the development of many human cancers. The tumor suppressor gene functions include regulation of the cell cycle and the cellular response to DNA damage, initiation of DNA repair and replication, induction of apoptosis and promotion of cell differentiation.

Case Report: Smears from ten cases of cervical lesions were analyzed for status of exons 5-8 of the p53 gene using PCR/SSCP.