Modulation of Zebrafish () Intestinal Mucosal Barrier Function Fed Different Postbiotics and a Probiotic from .

It is generally accepted that microbes play a critical role in maintaining gut barrier function, making them ideal to target in order to mitigate the effects of intestinal diseases such as inflammatory bowel disease with specialist supplementations such as probiotic or postbiotic preparations. In this study, specific strains of both live and inactivated and inactivated were fed to zebrafish at an inclusion level of 6 × 10 cells/g in order to assess the effects on gut barrier function and protection. Taken together, our results indicate that dietary administration of pro- or postbiotics strengthens the gut barrier function and innate immunity of healthy zebrafish in a strain-specific and process-dependent way.

Effects of yeast hydrolysate supplementation in low-fish meal diets for pikeperch.

Plant proteins have been increasingly used as sustainable substitutes for fish meal (FM) in aquafeeds; however, their high inclusion level compromises fish performance. The objective of this study was to examine whether yeast hydrolysate (YH) supplementation can improve the utilisation of high soybean meal (SM) diet and ameliorate its potential deteriorating impacts in pikeperch (Sander lucioperca). A basal diet was formulated using 44% FM, and four additional diets were produced by replacing 30 or 60% of FM with SM with or without the addition of 2% YH (FM, SM30, SM60, SM30 + YH, and SM60 + YH diets).

Yeast cell wall extracts from varying in structure and composition differentially shape the innate immunity and mucosal tissue responses of the intestine of zebrafish ().

With the rising awareness of antimicrobial resistance, the development and use of functional feed additives (FFAs) as an alternative prophylactic approach to improve animal health and performance is increasing. Although the FFAs from yeasts are widely used in animal and human pharma applications already, the success of future candidates resides in linking their structural functional properties to their efficacy . Herein, this study aimed to characterise the biochemical and molecular properties of four proprietary yeast cell wall extracts from in relation to their potential effect on the intestinal immune responses when given orally.

Effects of paraprobiotics on bile acid metabolism and liver health in largemouth bass () fed a cottonseed protein concentrate-based diet.

Cottonseed protein concentrate is a sustainable fishmeal alternative in aquafeed. A 10-week experiment was conducted to investigate the effects of a cottonseed protein concentrate-based diet with and without multi-strain yeast fractions (MsYF) on growth, bile acid metabolism, and health in largemouth bass. Four hundred fish (54.

Dietary Probiotic MA18/5M Improves the Growth, Feed Performance and Antioxidant Status of Penaeid Shrimp : A Growth-Ration-Size Approach.

Probiotics are increasingly documented to confer health and performance benefits across farmed animals. The aim of this study was to assess the effect of a constant daily intake of the single-strain probiotic MA18/5M (4 × 10 CFU.day.

Electrochemical sulfonylation of imidazoheterocycles under batch and continuous flow conditions.

An efficient and versatile protocol for the C-H sulfonylation of imidazoheterocycles electrochemical activation was established under batch and flow conditions. The selective C-H bond functionalization proceeded under catalyst- and oxidant-free conditions and tolerated a wide range of functional groups. Various sodium sulfinates as well as imidazo[1,2-]-pyridines, -pyrimidine, -quinolines, and -isoquinolines, imidazo[1,2-]pyridazine, imidazo[2,1-]thiazoles and benzo[]imidazo[1,2-]thiazoles reacted successfully.

Characterizing the interactions between classical and community-aware centrality measures in complex networks.

Identifying vital nodes in networks exhibiting a community structure is a fundamental issue. Indeed, community structure is one of the main properties of real-world networks. Recent works have shown that community-aware centrality measures compare favorably with classical measures agnostic about this ubiquitous property.

A novel dietary multi-strain yeast fraction modulates intestinal toll-like-receptor signalling and mucosal responses of rainbow trout (Oncorhynchus mykiss).

This study was conducted to evaluate the mucosal immune responses of rainbow trout when supplementing an experimental formulated feed with multi-strain yeast fraction product (Saccharomyces cerevisiae and Cyberlindnera jardinii). In total, 360 fish (initial BW 23.1 ± 0.

Use of Lactic Acid Bacteria During Pikeperch () Larval Rearing.

This trial tested the use of lactic acid bacteria (LAB) on pikeperch () larvae during their first feeding. The trial included the use of two probiotic treatments and one control (no probiotics). Pikeperch larvae were exposed to LAB as follows: (1) the live feed (Treatment 1, live feed) or (2) via the live feed and the larval rearing water (Treatment 2, probiotic).

Blood anti-Müllerian hormone is a possible determinant of recurrent early miscarriage, yet not conclusive in predicting a further miscarriage.

Research Question: Is blood anti-Müllerian hormone (AMH) concentration a strong determinant of unexplained recurrent early miscarriage (REM)?

Design: In the first part of the study, AMH concentrations measured using an Immunotech ELISA Kit were compared between 188 unselected (mostly fertile) women consecutively referred for three or more miscarriages in the first trimester of pregnancy and 376 age-matched parous women without pregnancy loss. Cases and controls were previously enrolled in an incident case-control study on thrombophilic mutations. Blood samples were collected >2 months after any recognized obstetric event or hormonal treatment.

Interventions for promoting participation in shared decision-making for children with cancer.

Background: This is an update of the Cochrane systematic review of shared decision-making (SMD) making published in 2013. Children's rights to have their views heard in matters that affect their lives are now well established since the publication of the UN Convention treaty (1989). Children with cancer generally prefer to be involved in decision-making and consider it important that they have the opportunity to take part in decision-making concerning their health care, even in end-of-life decisions.

Isolation, identification and characterisation of ballan wrasse Labrus bergylta plasma pigment.

This study confirmed that observations of blue-green colouration in plasma fractions of the ballan wrasse Labrus bergylta were caused by the linear tetra-pyrrole biliverdin and that the molecule was of the physiologically relevant IXα isomer. Accumulation appears driven by chromogenic association with an unknown protein moiety which precludes enzymatic reduction and would suggest active management. It was demonstrated that the pigment did not fluctuate relative to ontogeny, or indeed binary gender in the species of interest, but mobilisation and depletion in the subset of individuals undergoing sex change at the time of study supports a potential association with gender inversion processes.

Information fusion-based approach for studying influence on Twitter using belief theory.

Influence in has become recently a hot research topic, since this micro-blogging service is widely used to share and disseminate information. Some users are more able than others to influence and persuade peers. Thus, studying most influential users leads to reach a large-scale information diffusion area, something very useful in marketing or political campaigns.

eClims: An Extensible and Dynamic Integration Framework for Biomedical Information Systems.

Biomedical information systems (BIS) require consideration of three types of variability: data variability induced by new high throughput technologies, schema or model variability induced by large scale studies or new fields of research, and knowledge variability resulting from new discoveries. Beyond data heterogeneity, managing variabilities in the context of BIS requires extensible and dynamic integration process. In this paper, we focus on data and schema variabilities and we propose an integration framework based on ontologies, master data, and semantic annotations.

[The influence of age and illness duration on cognitive impairment in aging patients with relapsing-remitting multiple sclerosis (RR-MS)].

The aim of this study was to dissociate age and duration of illness effects on cognitive impairment of patients with relapsing-remitting multiple sclerosis. Cognitive impairment among patients with multiple sclerosis (MS) is well known. However, few studies were devoted to assess the respective role of disease duration and age on cognitive functions in MS patients.

Gender distribution, sexual size dimorphism and morphometric sexing in ballan wrasse Labrus bergylta.

In wild ballan wrasse Labrus bergylta, mass-length relationships were not different between genders, and positive allometry was found in the mixed-gender population. Male-biased sexual size dimorphism was significant and the most effective morphometric method for sexing L. bergylta outside of the species spawning window used body mass (M(B) in g), total body length (L(T) in mm) and Fulton's condition factor (K) as discriminant variables to predict gender with 91% accuracy.

Serum 2-hydroxyglutarate production in IDH1- and IDH2-mutated de novo acute myeloid leukemia: a study by the Acute Leukemia French Association group.

Purpose: Mutated isocitrate dehydrogenases (IDHs) 1 and 2 produce high levels of 2-hydroxyglutarate (2-HG). We investigated whether, in acute myeloid leukemia (AML), serum 2-HG would predict the presence of IDH1/2 mutations at diagnosis and provide a marker of minimal residual disease (MRD).

Patients And Methods: Serum samples from 82 patients at diagnosis of de novo AML (IDH1/2 mutated, n = 53) and 68 patients without AML were analyzed for total 2-HG and its ratio of D to L stereoisomers by mass spectrometry.

Delousing efficiency of farmed ballan wrasse (Labrus bergylta) against Lepeophtheirus salmonis infecting Atlantic salmon (Salmo salar) post-smolts.

Background: Cleaner-fish (wrasse, Labridae) are increasingly deployed within the Atlantic salmon (Salmo salar L.) industry as a biological control against sea-lice (Lepeophtheirus salmonis Krøyer). Two tank-based trials were performed to test the effect of farmed ballan wrasse (Labrus bergylta Ascanius) body mass and supplementary feeding on the delousing of Atlantic salmon post-smolts with an initial infection level of ∼12 lice salmon(-1) and a ∼5% wrasse:salmon ratio.

The volume effect in paediatric oncology: a systematic review.

Background: For several adult cancer types, there is evidence that treatment in high volume hospitals, high case volume providers, or in specialised hospitals leads to a better outcome. The aim of this study is to give an overview of the existing evidence regarding the volume effect in paediatric oncology related to the quality of care or survival.

Materials And Methods: An extensive search was carried out for studies on the effect of provider case volume on the quality of care or survival in childhood cancer.

Validation of search filters for identifying pediatric studies in PubMed.

Objective: To identify and validate PubMed search filters for retrieving studies including children and to develop a new pediatric search filter for PubMed.

Study Design: We developed 2 different datasets of studies to evaluate the performance of the identified pediatric search filters, expressed in terms of sensitivity, precision, specificity, accuracy, and number needed to read (NNR). An optimal search filter will have a high sensitivity and high precision with a low NNR.

The potential utility of the leopard pleco (Glyptoperichthys gibbiceps) as a biological control of the ciliate protozoan Ichthyophthirius multifiliis.

Background: Infections of the ciliate protozoan Ichthyophthirius multifiliis Fouquet, 1876, can cause heavy mortalities in freshwater aquaculture systems. Following the ban of malachite green on fish for human consumption, currently available chemical therapies are only partially efficacious even when repeatedly applied. There is, therefore, an urgent necessity to identify more efficient and environmentally friendly control strategies.