A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures.

In recent years, many biological-based products have been developed, representing a significant fraction of income in the pharmaceutical market. Ion exchange chromatography is an important downstream step for the purification of target recombinant proteins present in clarified cell extracts, together with many other unknown impurities. This work develops a robust approach to model and simulate the purification of untagged heterologous proteins, so that the improved conditions to carry out an ion exchange chromatography are identified in a rational basis prior to the real purification run itself.

Modeling and simulation of anion exchange chromatography for purification of proteins in complex mixtures.

Ion exchange chromatography is extensively used in the purification of biological compounds. Reliable mathematical models describing this chromatographic technique are available and can be used to improve the performance of this separation step. However, the use of synthetic mixtures for model development hampers the application of this approach with real cell extracts processed in downstream operations.

Effects of soy peptone on the inoculum preparation of Streptococcus zooepidemicus for production of hyaluronic acid.

Soy peptone (SP) was studied as nutrient source in replacement of the conventional media as Brain-Heart Infusion (BHI) and sheep blood in the first seed culture medium in Petri plates of Streptococcus zooepidemicus. This substitution, aimed at meeting the claim of the pharmaceutical and cosmetics industries, for the removal of animal sources of the culture media used in obtaining their products for safety reasons. The animal sources were used as a control.