Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing.

Nanosecond pulsed electric field (nsPEF) processing is gaining momentum as a physical means for single-cell bioconversion efficiency enhancement. The technology allows biomass yields per substrate (Y) to be leveraged and poses a viable option for stimulating intracellular compound production. NsPEF processing thus resonates with myriad domains spanning the pharmaceutical and medical sectors, as well as food and feed production.

Automated Online Flow Cytometry Advances Microalgal Ecosystem Management as , High-Temporal Resolution Monitoring Tool.

Microalgae are emerging as a next-generation biotechnological production system in the pharmaceutical, biofuel, and food domain. The economization of microalgal biorefineries remains a main target, where culture contamination and prokaryotic upsurge are main bottlenecks to impair culture stability, reproducibility, and consequently productivity. Automated online flow cytometry (FCM) is gaining momentum as bioprocess optimization tool, as it allows for spatial and temporal landscaping, real-time investigations of rapid microbial processes, and the assessment of intrinsic cell features.

Nanosecond pulsed electric field processing of microalgae based biorefineries governs growth promotion or selective inactivation based on underlying microbial ecosystems.

Nanosecond pulsed electric field treatment (nsPEF) is a technology-driven, resource-efficient approach fostering microalgae biorefineries for transforming them into economically viable scenarios. A processing window of 100 ns, 7 Hz, and 10 kV cm significantly leveraged phototrophic Chlorella vulgaris and bacterial counts up to + 50.1 ± 12.

Perspective on Pulsed Electric Field Treatment in the Bio-based Industry.

The bio-based industry is urged to find solutions to meet the demands of a growing world population. In this context, increased resource efficiency is a major goal. Pulsed electric field (PEF) processing is a promising technological solution.

Continuous nanosecond pulsed electric field treatments foster the upstream performance of Chlorella vulgaris-based biorefinery concepts.

Nanosecond pulsed electric field treatment (nsPEF) is an innovative, technology-driven, and resource-efficient approach to foster the upstream performance of microalgae-based biorefinery concepts to transform microalgae into economic more viable raw materials for the biobased industry. A processing window applying three treatments of 100 ns, 5 Hz, and 10 kV cm to industrially relevant phototrophic Chlorella vulgaris in the early exponential growth phase significantly increased biomass yields by up to 17.53 ± 10.

Pulsed electric field based cyclic protein extraction of microalgae towards closed-loop biorefinery concepts.

Microalgae-based biorefinery concepts can contribute to providing sufficient resources for a growing world population. However, the performance needs to be improved, which requires innovative technologies and processes. Continuous extraction from Chlorella vulgaris cultures via pulsed electric field (PEF) processing might be a viable process to increase the performance of microalgae-based biorefinery concepts.

Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae.

Photoautotrophic microalgae based biorefinery concepts are currently not competitive compared to other established production systems. Therefore, innovative upstream processes need to be developed to increase the competitiveness of photoautotrophic microalgae biorefinery concepts. Abiotic sub-lethal stress induction via nanosecond pulsed electric field (nsPEF) treatment might be a viable process to increase the efficiency of photoautotrophic microalgae cultivation.

Comprehensive pulsed electric field (PEF) system analysis for microalgae processing.

Pulsed electric field (PEF) is an emerging nonthermal technique with promising applications in microalgae biorefinery concepts. In this work, the flow field in continuous PEF processing and its influencing factors were analyzed and energy input distributions in PEF treatment chambers were investigated. The results were obtained using an interdisciplinary approach that combined multiphysics simulations with ultrasonic Doppler velocity profiling (UVP) and rheological measurements of Arthrospira platensis suspensions as a case study for applications in the biobased industry.