MDC1 maintains active elongation complexes of RNA polymerase II.

The role of MDC1 in the DNA damage response has been extensively studied; however, its impact on other cellular processes is not well understood. Here, we describe the role of MDC1 in transcription as a regulator of RNA polymerase II (RNAPII). Depletion of MDC1 causes a genome-wide reduction in the abundance of actively engaged RNAPII elongation complexes throughout the gene body of protein-encoding genes under unperturbed conditions.

Differential protein occupancy profiling of the mRNA transcriptome.

Background: RNA-binding proteins (RBPs) mediate mRNA biogenesis, translation and decay. We recently developed an approach to profile transcriptome-wide RBP contacts on polyadenylated transcripts by next-generation sequencing. A comparison of such profiles from different biological conditions has the power to unravel dynamic changes in protein-contacted cis-regulatory mRNA regions without a priori knowledge of the regulatory protein component.

[Cancer in esophagus and cardia--incidence trends in Denmark].

Introduction: The incidence of adenocarcinoma in esophagus and cardia has been reported as increasing in a number of countries over recent decades. We examined if a similar increase has occurred in Denmark. The study evaluates the incidence trends for esophagus- and stomach cancer in the period 1943-2003 with focus on changes since 1978.