[Effects of miR-31-5p on HIF-1α/BNIP3 signaling pathway and the expression of osteoblast-related factors of dental pulp stem cells].

Purpose: To investigate the effects of microRNA-31-5p (miR-31-5p) on the signal pathway of hypoxia inducible factor-1α (HIF-1α)/Bcl-2/adenovirus E1B 19-kDa-interacting protein 3(BNIP3) and the expression of osteoblast-related factors of dental pulp stem cells(DPSCs).

Methods: Human dental pulp stem cells (DPSCs) were cultured in vitro and divided into the control group (no transfection), mimic NC group (transfected with negative control-miR-31-5p), miR-31-5p mimic group (transfected with hsa-miR-31-5p mimic), siRNA NC group (transfected with nonsense siRNA) and miR-31-5p siRNA group (transfected with miR-31-5p siRNA).The expressions of miR-31-5p, HIF-1α, BNIP3, alkaline phosphatase(ALP) and Runt-related transcription factor-2(Runx2) mRNA in DPSCs were detected by real-time fluorescence quantitative PCR; the proliferation of DPSCs was detected by MTT; ALP activity of DPSCs was detected by ALP activity test kit; and the protein expressions of HIF-1α, BNIP3 and Runx2 in DPSCs were detected by Western blot.

[Change of zygomatic and temporal soft tissue after coronal incision].

Objective: To investigate the change of zygomatic and temporal soft tissue after coronal incision.

Methods: A retrospective analysis was performed in 33 patients who received firm fixation for unilateral zygomatic comminuted fracture through semi-coronal incision. All the patients were followed up for more than one year.

[Expression and significance of vascular cell adhesion molecule-1 in human oral squamous cell carcinoma].

Objective: To study the expression and the location of vascular cell adhesion molecule-1 (VCAM-1) gene and its clinical significance in human oral squamous cell carcinoma (OSCC).

Methods: In situ hybridization, PV-9000 polymer detection system for immunohistochemical staining was used to detect the expression and the location of VCAM-1 mRNA and VCAM-1 protein in 48 cases of OSCC and 10 cases of normal controls. Statistical analysis was performed using chi-square test in SPSS 13.

[Correlations between the expression of vascular cell adhesion molecule-1 gene and clinical pathological characteristics and angiogenesis in oral squamous cell carcinoma].

Purpose: To investigate the correlations between the expression of vascular cell adhesion molecule-1 (VCAM-1) gene and clinicopathologic characteristics and microvessel density (MVD) in oral squamous cell carcinoma (OSCC).

Methods: Expression and location of VCAM-1mRNA and protein in 48 OSCCs and 10 normal controls were detected by in situ hybridization and immunohistochemical stainingìMVD was also assessed. Statistical analysis was performed using SPSS13.

[Influence of prostaglandin E2 on proliferation of melanocytes in full-thickness skin graft].

Objective: To investigate the influence of the prostaglandin E2 on the proliferation of the melanocytes in the full-thickness skin graft.

Methods: Sixty-eight guinea-pigs were divided into experimental-1 group (skin graft), experimental-2 group (skin graft + diclofenac), and control groups. After the full-thickness skin graft, the dynamic changes of the prostaglandin E2 were measured and the proliferation of the melanocyte with its density was also evaluated by using histochemical and autoradiographic methods.