[The Establishment and Application of Flow Cytometry on the Detection of Jka Antigen in Kidd Blood Group].

Objective: To establish a based method flow cytometry to identify the antigen Jka in human red blood cells (RBCs) and verify its accuracy.

Methods: A total of 96 blood samples were enrolled in the study randomly from the voluntary blood donors in Shenzhen Blood Center. The RBCs were incubated with IgG anti-Jka primary antibody, and then labeled with the secondary antibody anti-IgG-Alexa Fluor 647.