Publications by authors named "Le P Ngo"
CometChip enables parallel analysis of multiple DNA repair activities.
DNA Repair (Amst)
October 2021
Article Synopsis
- DNA damage is harmful and linked to aging, cancer, and diseases, prompting cells to develop effective DNA repair mechanisms.
- Traditional DNA repair assays are often low throughput and only examine a single protein or repair pathway, limiting their effectiveness.
- The CometChip platform enhances the traditional comet assay by enabling simultaneous analysis of multiple DNA repair activities, proving effective for different repair pathways and increasing overall sensitivity and throughput.
Article Synopsis
- DNA repair is important for preventing diseases like cancer, but studying it in many people is tough.
- Researchers used a special test called CometChip to examine how quickly different people can repair their DNA after damage caused by a substance called HO.
- They discovered that some people repair their DNA faster than others, with the slowest taking about 81 minutes and the fastest only needing about 24 minutes, showing potential for future health studies.
Article Synopsis
- Genotoxicity testing helps scientists figure out if chemicals from drugs, industries, or the environment can harm DNA and cause cancer.
- The alkaline comet assay is a common test that finds DNA breaks, but it can miss certain harmful changes called bulky adducts.
- To detect these missed changes, researchers use special cells and new technology called HepaCometChip, which helps spot these dangerous DNA issues better than older tests.
Cell survival is a critical and ubiquitous endpoint in biology. The broadly accepted colony formation assay (CFA) directly measures a cell's ability to divide; however, it takes weeks to perform and is incompatible with high-throughput screening (HTS) technologies. Here, we describe the MicroColonyChip, which exploits microwell array technology to create an array of colonies.
View Article and Find Full Text PDFIn vivo measurements of interindividual differences in DNA glycosylases and APE1 activities.
Proc Natl Acad Sci U S A
November 2017
The integrity of our DNA is challenged with at least 100,000 lesions per cell on a daily basis. Failure to repair DNA damage efficiently can lead to cancer, immunodeficiency, and neurodegenerative disease. Base excision repair (BER) recognizes and repairs minimally helix-distorting DNA base lesions induced by both endogenous and exogenous DNA damaging agents.
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