Targeted Killing of Staphylococcus aureus Using Specific Peptides Displayed on Yeast Vacuoles.

Staphylococcus aureus is a common pathogen that causes health care-related and community-associated infections. In this study, we provide a novel system that can recognize and kill S. aureus bacteria.

Removing ammonium from contaminated water using Purolite C100E: batch, column, and household filter studies.

Ammonium removal from drinking water to protect human and environmental health is one of the major global concerns. This study evaluates the performance of Purolite C100E, a commercial cation exchange resin, in eliminating ammonium in synthetic and real contaminated groundwater. The results demonstrate that the pH operation range of the resin for better ammonium removal is 3 to 8.

Effect of acid trehalase (ATH) on impaired yeast vacuolar activity.

In this study, this protein was overexpressed in yeast cells grown on trehalose-containing medium to assess its impact on yeast vacuolar activity. ATH was confirmed to be located in both cell surface and vacuoles and the overexpression of ATH was observed to decrease vacuolar activity. Therefore, an assumption was suggested to explain this phenomenon as follows: when grown on containing trehalose medium, the ATH localization at cellular periplasm, but not the vacuole, is prioritized to utilize the extracellular trehalose for cell growth.

Effect of GTP-binding protein (YPT1 protein) on the enhanced yeast vacuolar activity.

Yeast GTP-binding protein (YPT1 protein) has been reported to function in the early stages of the secretory pathway. Particularly, YPT1 protein is observed to regulate both the endoplasmic reticulum-to-Golgi transport and the autophagy. Therefore, the YPT1 protein overexpressed in yeast vacuoles is expected to enhance antimicrobial and anticancer activity.