Metabolic alkalosis in skilled nursing patients.

Renal failure is common among the long-term care (LTC) elderly. Little is known about the acid/base status of these patients. The aim of this study is to evaluate the relationship between the acid base status and renal function in a representative group of skilled nursing patients and relate it to their feeding status.

[Magnetic resonance tomography in the diagnosis of the neurological complications of lumbar osteochondrosis].

The analysis of results of magnetic resonance tomography was performed in 289 patients with neurological complications of lumbar osteochondrosis. Among such patients radicular and reflectoral syndromes were observed in 184 patients while diskogenic lumbar-sacral radiculomyeloischemias--in 105 ones. The high informativity of the method was demonstrated in visualisation of localisation, direction and sizes of intervertebral disks' hernias at lumbar osteochondrosis as well as in establishment of such factors which predisposed diskogenic radiculomyeloischemias' development.

[Rotenone-sensitive oxidation of NADH and F0F1-ATPase activity in a homogenate of rat skeletal muscles during thermal adaptation].

A method has been developed for measuring the rates of rotenone-sensitive oxidation of NADH and oligomycin-sensitive hydrolysis of ATP in rat skeletal muscle homogenates. The method is based on the use of alamethicin which increases the permeability of the inner mitochondrial membrane for NADH and ATP. It has been shown that prolonged cold adaptation of rats (4 weeks, 4 degrees) does not change the activity of rotenone-sensitive NADH-oxidase in rat skeletal muscle homogenates which is equal to 12.

[Nuclear magnetic tomography in the diagnosis of spinal cord cysts].

Magnetic resonance tomography (MRT) was used in examinations of 172 patients with spinal cord abnormalities. Thirty-eight cysts were detected: 25 in syringomyelia, 5 in intramedullary tumors, 4 posttraumatic and 4 postoperational ones. Based on the MRT patterns, two types of syringomyelia were distinguished, the occlusive and idiopathic ones.

[The production of autoantibodies to nerve tissue glycolipid antigens in patients with traumatic spinal cord injuries].

The production of autoantibodies (AAB) to the specific antigen of myelin-containing components of the nervous tissue galactocerebroside (GalC) was revealed in 117 patients with traumatic injuries to the spinal cord of various severity in periods of 18 months to 46 years after the injury. The degree of nervous system destruction, the type of the course of posttraumatic myelopathy, and the level of production of AAB to GalC were found interrelated. The existence of AAB provides evidence of sensitization of the immune system to nervous tissue antigens in spinal cord injury, while the level of AAB to GalC reflects the activity of the continuing destructive process due to the injuring effect of AAB under conditions of impaired permeability of the blood-brain barrier and shows the desirability of desensitization therapy.

[Ossification of the posterior longitudinal ligament].

The authors described 21 patients aged 37 to 83 with ossification of the posterior longitudinal ligament (10 with ischemic myelopathy, 6 with aftermaths of a trauma of the cervical spine, and 5 with the radicular syndrome on the basis of vertebral osteochondrosis. X-ray signs of three types of ossification of the posterior longitudinal ligament were defined and specified: linear, curved and mixed. The linear type was more frequently observed in the cervical spine whereas the curved type was more frequent in the lumbar spine.

[Immobilization of EcoR1, Pae1 and Lpl1 restriction enzymes].

In search for sorbents (silica gels, styrene-divinylbenzene copolymers), for immobilization of some restriction endonucleases, derivatives of trityl-containing silochroms are shown to bind EcoRI, PaeI and LplI endonucleases with the retention of 10-20, 60-70 and 40-60% activity, respectively. The immobilized restriction endonucleases have the unchanged substrate specificity, can be used several times and are stable at storage. Tritylaminopropylsilochrom is suggested to be the sorbent of choice.

[Status of liver mitochondria and rat tissue creatine kinase during administration of antivitamin K].

It has been shown that 16-18 days administration of antivitamin K (pelentan) leads to two-fold increase of prothrombin time in adult rats but does not influence the soluble brain, renal, heart, muscle and serum creatine kinase activity. No effect on metabolic function of isolated liver mitochondria has been found in contrast to the vitamin K deficient rats. Mitochondria were characterized by high value of respiration control; substance oxidation rates and internal mitochondrial Ca2+ content do not differ in the level from those of control animals.

[Metabolic function of isolated liver mitochondria during various conditions of vitamin D and K supply and administration of pelentane].

Alimentary deficiency of vitamin K caused a decrease in the rate of respiration in presence of ADP and in the rate of oxidative phosphorylation in the presence of succinate. Administration of the antivitamin K pelentane, excess of vikasol and deficiency of vitamin D did not affect these parameters. As distinct from controls and rats treated with pelentane, transport of calcium was decreased in presence of all the substrates studied in mitochondria isolated from liver tissue of animals deprived of vitamins K and D as well as of animals treated with vikasol excess.

[Autoantibodies to glycolipid antigens of oligodendroglia in acute and chronic disorders of spinal circulation].

Measurements were made of the titers of autoantibodies to antigenic markers of oligodendrocytes and myelin galactocerebrosides in the blood serum and cerebrospinal fluid from 29 patients with ischemic cerebrospinal insult and 100 patients with ischemic myelopathy. Acute and chronic disorders of spinal circulation were found to be associated with production of auto-antibodies to galactocerebrosides. The interrelationship was discovered between the fact of auto-antibody production and the degree of the recovery of deranged function (in poor recovery, auto-antibodies were preserved in the blood serum up to 3 years in diagnostically significant titers).

[The pseudotumorous course of ischemic myelopathies].

Study of 36 cases of ischemic myelopathy following a pseudotumorous course and 30 cases of spinal cord tumors revealed similarity and differences in the clinical manifestations which should be taken into account in differential diagnosis. Authentic differences in the content of serotonin and its metabolites in the cerebrospinal fluid and blood of this group of patients are pointed out.

[Ion-exchange hemosorption in the intensive therapy of liver insufficiency in patients with obstructive jaundice].

Hemosorption on thromboresistant ion-exchange resins synthetized at D. I. Mendeleev Moscow Chemical Technological Institute (MCTI) was used in combined therapy of hepatic failure.

[Effect of different types of interferon on the natural cellular cytotoxicity of lung cancer patients].

The natural killer cell cytotoxicity was studied in the 4 hour and 18 hour cytotoxic reaction with cultured human tumour cells (K562 and lung adenocarcinoma) labelled by 51Cr. The natural killer cell activity of cancer patients was lowered only in 4 hour cytotoxic reaction with target cells K562. The natural and recombinant leucocyte interferon and natural immune interferon stimulated in vitro the activity of natural killer cells in most of the experiments (80.

[Cytotoxic action of natural killer cells on human tumor cells].

Natural killer cell cytotoxicity was studied in a 18-hour 51Cr-release assay in the cultures of human tumor target cells: K562 leukemia and lung adenocarcinoma (LAC) cells. The mean cytotoxic value was similar for K562 and LAC cells: 36.13 +/- 3.

[Nature of endogenous proton conductance of the inner mitochondrial membrane. Role of Ca2+ transport system in proton transfer].

In order to elucidate the nature of endogenous proton conductance of rat liver inner mitochondrial membrane, the dependence of the rate of Ca2+ transport on pH was studied. It was found that the inhibiting effect of H+ is independent of protonation of functional groups of hypothetical Ca2+ carrier, but results from electrogenic transfer of H+ across the membrane, which is highly permeable for the proton. The adsorption of H+ by mitochondria is inhibited by ruthenium red and other specific inhibitors of Ca2+ transport.

[Role of planar cobalt chelate complexes as shunting agents in the electron transport chain of mitochondria].

It was shown that planar cobalt chelate complexes catalyze oxidation of NADH and hexahydroubiquinole Q4H2 by air oxygen. The most active agent--perchlorate of the cobalt complex 1, 2, 3, 7, 8, 12, 13, 17, 18, 19-decamethyloctadehydrocorrin--is capable of shunting the electron transfer chain of rat liver mitochondria and beef heart submitochondrial particles, catalyzing electron transfer from CoQ directly to the oxygen.