[Dermatoplasty in post-traumatic necrosis of soft tissues of the hand and fingers].

Early necrectomy followed by skin plasty allows preserving impaired parts of the hand and working capacity of the patients in posttraumatic necrosis of soft tissues of the hand and fingers. The method of skin plasty and time of its fulfilling should be dependent on the character of injuries, their localisation and degree of necrosis. In the absence of wound infection skin plasty must be fulfilled immediately after necrectomy.

[Secondary cicatricial deformities and recurrences after surgical interventions on the hand in congenital syndactylia].

Experience with the treatment of 46 patients with syndactylism of the hand is described. In 28 of them operations for liquidation of syndactylism were repeatedly performed in childhood. The operation technique and results of the treatment are presented.

[Changes in the static electric field in restrained animals].

The paper states that restraint of an animal causes transition from one quasistationary level of the static electric field to another.

[Basal phosphorylation of synaptic membrane proteins in the presence of ATP and GTP].

A comparative study of endogenous ATP- and GTP-used basal protein phosphorylation in synaptic membranes from the rat brain cortex was carried out using the method of determination of total protein phosphorylation and autoradiography of proteins separated in PAGE. Under identical conditions 33P incorporation into proteins was shown to be appropriate in the presence of ATP as contrast to that in the case of GTP; in both cases the same proteins were phosphorylated. Inhibition of endogenous basal phosphorylation by alkali cations (K+, Na+, Li+) was more effective in the presence of GTP as compared to that with ATP.

[GTP--a substrate for 3',5'-AMP-dependent phosphorylation of synaptic membrane proteins].

Using the method of electrophoresis in polyacrylamide gel (PAGE) it is shown that not only ATP, but GTP may play the role of phosphate donor for 3':5'-monophosphate (cAMP)-dependent phosphorylation. GTP is incorporated predominantly in the same "classic" 3':5'AMP-dependent phosphorylated proteins (with Mr 86 000, 82 000, 68 000, 61 000, 54 000) as that from ATP does. EGTA treatment of synaptic membranes enhances 3':5'-monophosphate (cAMP)-dependent phosphorylation with GTP used as a phosphate donor.

Calmodulin-dependent regulation of calcium-activated outward current in frog atrial membrane.

The steady-state outward current underlying the inward-going rectification in frog atrial fibers has been studied by the double sucrose gap technique. Similar to the case in sheep Purkinje fibers (18,19), the inward rectifying potassium channels in frog atrium are blocked by cesium (5 mM) and activated by increasing cytosolic calcium concentration (replacing 30% of the Na by sucrose). It was shown that trifluoperazine (5 x 10(-6) M), a widely used blocker of the Ca-calmodulin complex, inhibits the inward rectifying potassium channels.

Mechanism of plateau loss in rabbit papillary muscle after a short rest. Role of outward current.

Shortening of the first action potential plateau phase and potentiation of isometric contraction are simultaneously found in rabbit papillary muscle after 1 min of rest. The ionic nature of these events was investigated. Shortening of the plateau phase is not markedly prevented by activators of the slow inward current (5'-guanyl imidodiphosphate, 10(-5) M plus isoproterenol, 10(-7) M) but is completely eliminated by potassium current blockade with 4-aminopyridine (2 X 10(-4) M).

Role of neurotransmitter release and cyclic AMP-dependent membrane phosphorylation in low voltage myocardial automaticity.

Low voltage myocardial automaticity (LVA) was investigated by pharmacological modulations of the presynaptic and postsynaptic processes. The sensitivity of LVA both to inhibitor and stimulator of neurotransmitter release suggests its involvement in LVA genesis. Moreover, LVA is blocked by the inhibition of the cyclic AMP system, supporting the participation of the c-AMP-dependent membrane phosphorylation in calcium-mediated cardiac electrogenesis.