Earth field NMR with chemical shift spectral resolution: theory and proof of concept.

A new method for obtaining an NMR signal in the Earth's magnetic field (EF) is presented. The method makes use of a simple pulse sequence with only DC fields which is much less demanding than previous approaches in terms of the pulses' rise and fall times. Furthermore, it offers the possibility of obtaining NMR data with enough spectral resolution to allow retrieving high resolution molecular chemical shift (CS) information - a capability that was not considered possible in EF NMR until now.

High-sensitivity Q-band electron spin resonance imaging system with submicron resolution.

A pulsed electron spin resonance (ESR) microimaging system operating at the Q-band frequency range is presented. The system includes a pulsed ESR spectrometer, gradient drivers, and a unique high-sensitivity imaging probe. The pulsed gradient drivers are capable of producing peak currents ranging from ∼9 A for short 150 ns pulses up to more than 94 A for long 1400 ns gradient pulses.

Electron spin resonance micro-imaging of live species for oxygen mapping.

This protocol describes an electron spin resonance (ESR) micro-imaging method for three-dimensional mapping of oxygen levels in the immediate environment of live cells with micron-scale resolution(1). Oxygen is one of the most important molecules in the cycle of life. It serves as the terminal electron acceptor of oxidative phosphorylation in the mitochondria and is used in the production of reactive oxygen species.

Molecular diffusion in porous media by PGSE ESR.

Diffusion in porous media is a general subject that involves many fields of research, such as chemistry (e.g. porous catalytic pallets), biology (e.

ESR micro-imaging of LiNc-BuO crystals in PDMS: spatial and spectral grain distribution.

Microcrystals of lithium octa-n-butoxynaphthalocyanine (LiNc-BuO) in a bio-compatible and oxygen-permeable polymer matrix of poly-dimethyl-siloxane (PDMS) can be used for repetitive non-invasive imaging of oxygen in live specimens by means of mm-scale electron spin resonance (ESR) imaging. This probe denoted as "oxychip" was characterized by high-resolution mum-scale ESR microcopy to reveal the fine details of its spatial and spectral properties. The ESR micro-images of a typical oxychip device showed that while the spatial distribution of the microcrystals in the polymer is fairly homogenous (as revealed by optical microscopy), the ESR signal originates only from a very few dominant crystals.

Ex situ endorectal MRI probe for prostate imaging.

A unique ex situ MRI probe, which examines samples external to its geometry, is presented. The probe is intended to be used for imaging the prostate gland via an endorectal pathway. It has a semicylindrical shape with a length of 6 cm and typical diameter of approximately 3 cm.

ESR imaging in solid phase down to sub-micron resolution: methodology and applications.

Electron spin resonance microcopy (ESRM) is an imaging method aimed at the observation of paramagnetic species in small samples with micron-scale spatial resolution. At present, this technique is pursued mainly for biological applications at room temperature and in relatively low static magnetic fields. This work is focused on the use of ESRM for the measurement of solid samples.