Development and laboratory validation of duplex real-time PCR for simultaneous detection of Brucella and bovine alphaherpesvirus from clinical specimens.

A duplex real‑time PCR was developed and validated for the simultaneous detection of Brucella and bovine alphaherpesvirus‑1 (BoHV‑1) from bovine clinical specimens. The bcsp31 gene of Brucella and gB gene of BoHV‑1 were used as targets in the assay. The limit of detection for BoHV‑1 was 0.