Loop-mediated isothermal amplification assay detects multiple alleles of genes in and other Gram-negative bacteria despite primer-template mismatches.

The known intrinsic and polymorphic genes of were recently reported in other non- Gram-negative pathogens. Accurate detection of this potentially transferrable carbapenemase gene in the clinical setting is critical. This study developed a loop-mediated isothermal amplification (LAMP) assay targetting multiple alleles of genes.