Rat "first-wave" mature thymocytes: cycling lymphoblasts that are sensitive to activation-induced cell death but rescued by interleukin 2.

The sequential appearance of thymocyte subsets in rat ontogeny was studied using the surface markers CD4, CD8, and the alpha/beta T cell receptor (here referred to as TcR). It was noted that the first TcRhigh thymocytes, appearing around birth, are not resting lymphocytes but cycling blast cells. These "first wave" TcRhigh cells are medullary in location and predominantly of the CD4/CD8 "single-positive" phenotype.

Identification and characterization of rat T cell subpopulations expressing T cell receptors alpha/beta and gamma/delta.

Peripheral lymphoid organs of the rat were investigated for the presence of lymphocytes that expressed the pan-T cell markers CD5 and OX-52 but not the T cell receptor (TcR) alpha/beta. Two such populations were identified: 2% to 4% of lymphocytes in adult spleen, lymph nodes and peripheral blood are CD5+ TcR alpha/beta- and express the OX-52 antigen at the same density as TcR alpha/beta+ T-cells. About 90% of these cells are CD8+.

A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes.

mAb R73 detects a T cell-specific surface molecule consisting of two disulfide-linked subunits of 40 and 46 kD, respectively, on 97% of peripheral rat T cells, as defined by the OX-52 marker. Of the few OX-52+ R73- cells, none are CD4+ but many express the CD8 antigen known to be present on rat NK cells. mAb R73 is mitogenic for unseparated spleen cells and for purified T cells.

Analysis of CD3 and antigen receptor expression on T cell subpopulations of aged athymic mice.

The expression of the T cell antigen receptor on subpopulations of extrathymically generated T cells from athymic mice was investigated and compared to antigen receptor expression in normal mice. To this end, spleen and lymph node cells from 18 individual athymic BALB/c nu/nu mice between 6 and 12 months of age and from normal controls were enriched for T cells by nylon wool filtration. Expression of the following cell surface markers was analyzed by two-color flow cytometry: Thy-1, CD4, CD8, V beta 8 and CD3.

Establishment of a temperature-inducible cell line for human plasminogen activator (tissue-type) by transfection of monkey cells with expression constructs.

An expression construct for human tissue-type plasminogen activator (t-pA) cDNA [containing a simian virus 40 (SV40) origin of replication] was introduced into CV1, COS-7 and COSts2 cells; in the latter cell line the amount of functionally active large T antigen of SV40 is regulated by the temperature. In a transient system, the expression in COSts2 cells at the permissive temperature for large T antigen was improved sixfold compared to COS-7 cells. By cotransfection with a plasmid conferring resistance to G418 into COSts2 cells, a cell line (COSts2Glob t-pA) could be isolated with barely detectable expression of t-pA at the semi-permissive and non-permissive temperature and inducible secretion of t-pA at the permissive temperature.