Features of morfological changes in primary thyroid gland CTLL cultures of rats descendants prenatally exposed by radioisotopes of iodine-131.

Objective: to investigate morphological changes in the primary thyroid cell culture of rat infants whose parents were prenatally exposed by radioisotope iodine 131.

Materials And Methods: obtaining and culturing of thyroid tissue primary cell cultures of newborn rats, cytological (receipt and analysis of cell cultures agents for optical microscopy), biophysical (flow cytometry), statistics.

Results: It was shown that cells in thyroid primary culture of offspring rats prenatally exposed by radioisotopes of iodine 131 signs of destructive degenerative changes were observed mostly when animals of both sexes were irra diated.

Radiomodifying and antitoxic effect of natural polymineral substances on the viability of the cell line L929 under the combined exposure to ionizing radiation and ions of heavy metals.

Objective: to study the radiomodifying and antitoxic effect of natural polymineral substances on cell viability in vitro under the combined exposure to ionizing radiation and ions of heavy metals.

Materials And Methods: Research was performed on an inoculated proliferating culture of the L929 cell line. Water soluble salts of lead acetate, copper, nickel and chromium oxide were added to the cell line culture at concentra tions of 0.

Impact of gadolinium-157 containing nanoscale magnetosensitive composites on morfofunctional properties of cells in vitro.

Objective - to investigate the morphofunctional cells properties under the action of magneticallybased nanocomposites containing gadolinium-157. Materials and methods. Experimental studies are performed in cell culture line L929 Nanocomposites based on magnetite modified by functional amino groups chemically fixed by diethylenetriaminepentaacetic acid (DTPA) and gadolinium - (Fe3O4/γ-APS/DTPA-Gd) were studied (1), also by meso-2,3-dimercaptosuccinic acid (DMSA) - (Fe3O4/DMSA-Gd), which binds to the hydroxyl group of magnetite surface (2); gadolinium was adsorbed from a solution of gadolinium sulfate.

Features of biological effects in cell culture in the combined exposure to ionizing radiation and copper ions.

The objective of the work was to research the features of monoand combined impact of ionizing radiation and copper salts on cell viability in vitro. Materials and methods. The studies were performed on a passaged line L929 cell culture.

[Phospholipid composition of normal and transformed cells cultivated with N-acylethanolamines].

Effects of N-stearoylethanolamine (NSE), N-oleoilethanolamine (OEA) and mixture of more than 20 saturated and unsaturated N-acylethanolamines on phospholipids composition of normal and transformed fibroblasts in the culture were compared in the work. It was shown that cultivation of cells NSE decreases percent content of phosphatidylinositol (PI), phosphatidylserine (PS) and sphingomyeline (SM) - important mediators of cell signaling. Under the influence of OEA the level of SM decreases as well, but at the same time PI and PS levels increase.

[Influence of N-acylethanolamines on embryonic cell culture survival under the effect of irradiation].

The effects of the N-stearoylethanolamine (NSE), N-oleoylethanolamine (OEA) and N-acylethanolamine (NAE) mixture on the cell survival, apoptosis and activity of mitochondrial succinate dehydrogenase (SDG) and glycerophosphate dehydrogenase (GFDG) in embryonic cell culture under normal conditions and irradiation were compared in the work. It was shown, that all investigated NAE were able to modulate the proliferative activity of intact cells as well as irradiation-exposed cells in concentration-depended manner. The most pronounced effect was observed under the NAE mixture action.

[N-stearoylethanolamine inhibits the proliferation of transformed cells and modulates mitochondrial enzyme activity in normal and transformed cells].

The N-stearoylethanolamine (NSE) influence on the proliferation of different cell types and the activity of mitochondrial electron transport enzymes, succinatedehydrogenase (SDG) and glycerophosphate-dehydrogenase (GFDG), in transformed cells under the action of NSE was studied. The incubation of the cells of mouse leukemic lymphocyte cell line L1210 and transformed mouse fibroblasts L929 with NSE caused the inhibition of cell growth and decreased the survival level of cells, but this effect was not associated with apoptotic cell death. It was shown for the first time that NSE addition to the cultural medium decreased the SDG activity and increased the GFDG activity in L929 cells.

[The cytotoxic effect of heavy metals on an L-cell culture].

The dependence of some parameters of L-cells culture viability on different concentrations of heavy metals was studied. Considerable cytotoxic effect of low concentrations of nickel (0.025 mcg/ml) and lead (0.