EFFECTIVENESS OF THE PHOTON CAPTURE BEAM TECHNOLOGY AND PHOTODYNAMIC IMPACT ON MALIGNANT HUMAN CELLS ІN A RESTING STATE.

Objective: to investigate the structural and morphofunctional changes in test system of malignant (cell line A-549) human cells in a resting state exposed to X-rays in the presence of gadolinium-containing photon capture agent «Dotavist» and optical light (red spectrum) in combination with «Photolon» photosensitizer.

Methods: Passaged malignant human cell culture technology, X-ray and red light exposure, cytological and statistical methods.

Results: X-ray exposure at a dose of 10.

EFFICIENCY OF PHOTON CAPTURE BEAM TECHNOLOGY AND PHOTODYNAMIC IMPACT ON MALIGNANT AND NORMAL HUMAN CELLS IN VITRO.

Objective: to investigate the structural and morphofunctional changes in test systems of malignant (cell lineA549) and normal (stem fibroblasts) human cells exposed to X-rays in the presence of gadoliniumcontaining photon capture agent «Dotavist» and optical light (red spectrum) in combination with «Fotolon» photosensitizer.

Methods: The continuous cell culture of normal human fibroblasts and malignant human cells technology, X-ray and red light exposure, cytological and statistical methods.

Results: Effects of the two binary radiation technologies, namely the photon capture impact on malignant cells(human nonsmall cell lung cancer cells i.

SELECTION AND TESTING OF EXPERIMENTAL MODELS OF NORMAL AND MALIGNANT HUMAN CELLS IN VITRO AND EVALUATION OF THEIR SENSITIVITY RANGE TO THE NEUTRON/CAPTURE AND PHOTON/CAPTURE AGENTS AND PHOTOSENSITIZERS.

Objective: to investigate the structural and morpho-functional changes in test systems of malignant (A-549 cellline) and normal (fibroblasts of the 6th passage) human cells during incubation with gadolinium-containing pho-ton-capture agent «Dotavist» and photosensitizer «Fotolon».

Methods: The passaged (continuously interweaved) cell culture technique on normal human fibroblasts and malig-nant human cells; cytological, biophysical, statistical methods.

Results: The cytotoxic properties of «Dotavist» gadolinium-containing photon-capturing agent and «Photolon»photosensitizer in a wide range of concentrations (5, 10, 25, 50, 100 and 200 μl/ml) were studied by the morpho-functional characteristics (growth kinetics, proliferative and mitotic activity, presence of atypical cells) in the invitro test systems of malignant (non-small cell lung cancer cell line A-549) and normal (6th passage fibroblasts)human cells.

NEUROENDOCRINE EFFECTS OF PRENATAL IRRADIATION FROM RADIOACTIVE IODINE (review).

Background: Neuroendocrine effects of the prenatal radiation exposure from radioactive iodine in an event of nuclear power reactor accidents are a key issue in the field of radiation medicine and radiation safety because of a dramatic radiosensitivity of the developing organism.

Objective: Review of contemporary epidemiological, clinical and experimental data on neuroendocrine effects of prenatal exposure to 131I.

Object And Methods: Search in the PubMed/MEDLINE and Google Scholar abstract databases, along with a manual search for the relevant data sources.

Experimental evaluation of 2-merkaptobenztyazol radioprotective capacities on cell cultures test system.

Objective: To study the radioprotective properties of 2-merkaptobenztyazol on proliferating cell culture test system.

Materials And Methods: cytogenetic, cytological, statistics.

Results: Analysis of cytogenetic effects of 2-merkaptobenztiazol by frequency aberrations criteria and aberrant cells damage criteria showed that upon entire investigated range of reagent concentrations the frequency of aberrations was not significantly higher than the value of this indicator in control, but there was a tendency of reduction of mitotic activity of root meristem cells.

Antiproliferative, Apoptotic, and Autophagic Activity of Ranibizumab, Bevacizumab, Pegaptanib, and Aflibercept on Fibroblasts: Implication for Choroidal Neovascularization.

Purpose. Choroidal neovascularization (CNV) is one of the most common complications of retinal diseases accompanied by elevated secretion of vascular endothelial growth factor (VEGF). Intravitreal anti-VEGFs (ranibizumab, bevacizumab, pegaptanib, and aflibercept) can suppress neovascularization, decrease vascular permeability and CNV size, and, thereby, improve visual function.

Features of cellular effects under combined effects of nickel ions and ionizing radiation.

Unlabelled: Under current conditions the probability of simultaneous effects of radiation and chemical factors on biological objects increases, and that's why the issue of combined influence of factore different by their nature becomes progressively important. The objective was to study the cytotoxicity of nickel ions in a test-system of cell culture and to detect the cellular reactions under a combined impact of nickel ions and ionizing radiation.

Materials And Methods: Studies were performed on inoculated cell culture line L929.

[Modifying effect of low-intensive electromagnetic radiation on the irradiated cells].

The normalising effect of low-intensive electromagnetic radiation on the viability of cell culture of L929 line, irradiated with gamma-quantums of 137Cs in a wide dose range was demonstrated.

[Survival and proliferative activity of L-cells after irradiation with neutrons of different energies].

With L-cells exposed to neutrons and X-rays the RBE of fission spectrum neutrons (1.2 MeV) was 2.8, and that of high-energy neutrons (22 MeV), 1.

[Effect of fast neutrons on the process of muscle cell differentiation].

A study was made of the effect of fast neutron on differentiation of myoblasts in vitro. The results obtained indicate that irradiation inhibits the differentiation of myoblasts in culture. Irradiation with fast neutrons (1.