Expression of hindlimb abnormalities under rearing temperature effects during the larval development of the salamander Pleurodeles waltl (urodele amphibian).

Anomalie m.p. is a spontaneous and heritable hindlimb abnormality described earlier.

Genetic evidence for linkage with the Z and W sex chromosomes of two distinct couples of alleles controlling larval and postmetamorphic skin pigmentation in salamander.

In Pleurodeles waltl, progeny resulting from a cross between 2 individuals of the Z/W sexual genotype include 25% of W/W individuals, while those issued from crossing a Z/W neomale with a W/W thelygenous female include 50% of W/W individuals. W/W individuals can be identified through the peptidase-1 zymogram since, in P. waltl, this enzyme is controlled by codominant alleles which are linked to the sex chromosomes.

Sex-linked peptidase-1 patterns in Pleurodeles waltlii Michah (Urodele Amphibian): genetic evidence for a new codominant allele on the W sex chromosome and identification of ZZ, ZW and WW sexual genotypes.

In Pleurodeles waltlii, peptidase-1 is a dimeric enzyme which has been shown to be controlled by two codominant alleles, Pep-1A and Pep-1B (Ferrier et al., 1980, 1983), linked respectively to the Z and W sex chromosomes. The enzymatic patterns obtained after starch gel electrophoresis were used routinely to identify the sexual genotypes of animals reared in our laboratory.

Cell death and abnormalities in limb morphogenesis ofPleurodeles waltlii Michah. (urodela, amphibia) after nitrogen mustard treatment.

The effects of nitrogen mustard (methylbis-chlorethylamine) on limb organogenesis were studied inPleurodeles waltlii at different stages of fore and hind limb development.The effects of four different doses of nitrogen mustard (1.25; 2.

[Histochemical data on the first stages of the fore and hind limb development inPleurodeles waltlii Michah. (Urodela, amphibia)].

Localization of 4 dephosphorylating enzymes (alkaline phosphomonoesterase, acid phosphomonoesterase, nonspecific ATP-ase, and AMP-ase=5' nucleotidase) has been studied in both constituants of the fore- and hind limb buds inPleurodeles waltlii Michah. during the first stages of development.There is no specific enzymatic reaction in the bud epiderm; the negative or positive results concerning the four enzyme groups are observed in the whole epiderm of the larval body and not only in the limb bud epiderm: the epiderm is devoid of the alkaline phosphomonoesterases which hydrolyze sodiumβ-glycerophosphate and sodiumα-naphthyl phosphate, and of AMP-ase and acid sodiumβ-glycerophosphatase; it is rich in ATP-ase and acid naphthol AS/BI phosphatase).