Publications by authors named "Laurine Reset"

Article Synopsis
  • This study introduces a simplified two-dimensional supercritical fluid chromatography (SFC) system designed for enhanced peak transfer in various modes, utilizing diode-array and mass spectrometric detection.
  • The system features a single SFC instrument augmented with three external valves, enabling precise peak parking and transfer between dimensions, regardless of mobile phase composition.
  • The effectiveness of the system is validated through the analysis of flavonoids in a natural product extract, showcasing excellent repeatability and high transfer rates between dimensions, which allows for accurate quantification of diastereomers.
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When starting a method development in supercritical fluid chromatography (SFC), the first step is usually to screen several stationary phases based on previous experience or simply based on what is available in the laboratory. However, as there are now a large number of stationary phases available for SFC, the choice of an adequate set of columns to rapidly achieve a satisfying result can be difficult. In this project, 16 columns comprising a wide diversity of stationary phases and polarities ranging from the most polar (like bare silica gel) to the least polar (like octadecylbonded-silica) were compared, based on the gradient analysis of 129 probe compounds.

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