Detection of foot-and-mouth disease virus in the breath of infected cattle using a hand-held device to collect aerosols.

Exhaled air of individual cattle infected experimentally with foot-and-mouth disease virus (FMDV) was sampled to assess the feasibility of a rapid, non-invasive general screening approach for identifying sources of FMDV infection. The air sampler used was a handheld prototype device employing electrostatic particle capture in a microchip chamber of 10-15 μL and was shown to effectively capture a high percentage of airborne microorganisms. The particles were eluted subsequently from the chip chamber and subjected to real-time RT-PCR.

Development and evaluation of novel one-step TaqMan realtime RT-PCR assays for the detection and direct genotyping of genogroup I and II noroviruses.

Background: Current detection and genotyping methods of genogroup (G) I and II noroviruses (NoVs) consist of a 2-step approach including detection of viral RNA by TaqMan realtime RT-PCR (RT-qPCR) followed by conventional RT-PCR and sequencing of partial regions of ORF1 or ORF2.

Objective: To develop novel long-template one-step TaqMan assays (L-RT-qPCR) for the rapid detection and direct genotyping of GI and GII NoVs and to evaluate the sensitivity and specificity of the assays.

Study Design: GI and GII-specific broadly reactive L-RT-qPCR assays were developed by combining existing NoV primers and probes targeting the open reading frame (ORF)1-ORF2 junction as well as region C at the 5'-ORF2.

[Rabies in a cat in Greenland].

We describe the first case of rabies diagnosed in a cat in Greenland. The cat showed aggressive behaviour one month after the visit of a rabid fox on the premises. Rabies is enzootic in Greenland, the arctic fox being the natural host of rabies virus.