Spatial organization of lysosomal exocytosis relies on membrane tension gradients.

Lysosomal exocytosis is involved in many key cellular processes but its spatiotemporal regulation is poorly known. Using total internal reflection fluorescence microscopy (TIRFM) and spatial statistics, we observed that lysosomal exocytosis is not random at the adhesive part of the plasma membrane of RPE1 cells but clustered at different scales. Although the rate of exocytosis is regulated by the actin cytoskeleton, neither interfering with actin or microtubule dynamics by drug treatments alters its spatial organization.

Circular Dichroism Measurement of Single Metal Nanoparticles Using Photothermal Imaging.

Circular dichroism (CD) spectroscopy is a powerful optical technique for the study of chiral materials and molecules. It gives access to an enantioselective signal based on the differential absorption of right and left circularly polarized light, usually obtained through polarization analysis of the light transmitted through a sample of interest. CD is routinely used to determine the secondary structure of proteins and their conformational state.

Spondylolisthesis in children under 12 years of age: long-term results of 56 patients treated conservatively or operatively.

Thirty-six girls and 20 boys who were diagnosed with spondylolisthesis of L5 when aged less than 12 years (10 months to 11 years) were reexamined clinically and radiologically at skeletal maturity, 5-30 years (average 14.5 years) later. Thirty-two children were treated operatively: 20 patients by posterior, 10 by posterolateral, and one child by anterior fusion.

Intertrochanteric varus osteotomy in the treatment of Perthes' disease.

Seventy-eight hips with Perthes' disease (LCPD) were treated with an intertrochanteric varus osteotomy. The hips were preoperatively graded according to Catterall's classification. There were 7 Group II, 32 Group III, and 39 Group IV hips.

Total hip replacement by the Brunswik prosthesis. A preliminary report of 189 operations.

The results of 189 Brunswik total hip replacements, performed at the Orthopaedic Hospital of the Invalid Foundation, Helsinki, Finland, and followed up for 2-3 years, are presented. According to the modified system of Merle D'Aubigne & Postel excellent results were achieved in 11.1 per cent (21/189), good in 56.

Results of operative treatment of lumbar disc herniation. A survey of 886 patients.

Over a period of 6 years (1960-65), 886 patients were operated on for herniated lumbar disc. In 1971, 695 of these patients (78.5 per cent) answered a questionnaire.

Operative treatment of spondylolisthesis in young patients.

In a series of 91 patients under 20 years of age with lytic spondylolisthesis treated by different operations, 66 (73%) showed a displacement of more than 30 per cent at admission. If the displacement exceeds 30 per cent, it often progresses to a subtotal olisthesis. Spinal fusion should be performed before the displacement exceeds a third of the length of the vertebra.

Late results of removal of the loose posterior element (Gill's operation) in the treatment of lytic lumbar spondylolisthesis.

This study presents the late results of the Gill operation for the treatment of lytic lumbar spondylolisthesis in 75 patients. The observation time ranged for 5 to 20 years, averaging 12 years. Primary results were excellent, good or fair in 83 per cent at the end of 1 year.

A comparison of dicloxacillin and ampicillin in the antibiotic prophylaxis of total hip replacement.

The effect of two semi-synthetic penicillins, dicloxacillin and ampicillin was compared in systemic antibiotic prophylaxis in total hip replacement patients. The drugs were given in routine oral dosage for eight days to two patient groups each consisting of 50 patients. There was no fatality.

A comparison of dicloxacillin and ampicillin in the antibiotic prophylaxis of total hip replacement.

The effect of two semi-synthetic penicillins, dicloxacillin and ampicillin was compared in systemic antibiotic prophylaxis in total hip replacement patients. The drugs were given in routine oral dosage for eight days to two patient groups each consisting of 50 patients. There was no fatality.

Femoral nerve compression syndrome with paresis of the quadriceps muscle caused by radiotherapy of malignant tumours. A report of four cases.

Four patients showed signs of femoral nerve compression with subsequent paresis of the quadriceps muscle, after radiation therapy of malignant tumours. The compression was caused by scar tissue due to radiation treatment of the inguinal region. The first symptom was radiating pain in the front of the thigh and lower leg which appeared 12-16 months after X-ray treatment.