Publications by authors named "Laurent Heliot"

Article Synopsis
  • The paper discusses the use of plasmonic photothermal therapy for targeting uropathogenic bacteria using reduced graphene oxide and gold nanorods/rGO composites.
  • An expression of concern has been published, addressing potential issues related to the methodology or data integrity in the research.
  • Researchers are encouraged to critically review the findings and verify the results to ensure scientific accuracy and reliability.
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Article Synopsis
  • The article discusses the development of gold nanorods with a silica core-shell structure, which are enhanced with verteporfin, a photosensitizer used in photodynamic therapy.
  • It emphasizes the high effectiveness of these nanostructures in the photodynamic inactivation of targeted cells or pathogens.
  • The authors express concern regarding potential issues or limitations identified in the study, which may impact the reproducibility or applicability of the findings in clinical settings.
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Expression of concern for 'Particle-based photodynamic therapy based on indocyanine green modified plasmonic nanostructures for inactivation of a Crohn's disease-associated strain' by Roxana Jijie , , 2016, , 2598-2605, https://doi.org/10.1039/C5TB02697K.

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Gene expression orchestration is a key question in fundamental and applied research. Different models for transcription regulation were proposed, yet the dynamic regulation of RNA polymerase II (RNAP II) activity remains a matter of debate. To improve our knowledge of this topic, we investigated RNAP II motility in eukaryotic cells by combining single particle tracking (SPT) and fluorescence correlation spectroscopy (FCS) techniques, to take advantage of their different sensitivities in order to analyze together slow and fast molecular movements.

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Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to probe the molecular environment of fluorophores. The analysis of FLIM images is usually performed with time consuming fitting methods. For accelerating this analysis, sophisticated deep learning architectures based on convolutional neural networks have been developed for restrained lifetime ranges but they require long training time.

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ERK1/2 involvement in cell death remains unclear, although many studies have demonstrated the importance of ERK1/2 dynamics in determining cellular responses. To untangle how ERK1/2 contributes to two cell death programs, we investigated ERK1/2 signaling dynamics during hFasL-induced apoptosis and TNF-induced necroptosis in L929 cells. We observed that ERK1/2 inhibition sensitizes cells to apoptosis while delaying necroptosis.

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Control of cell death/survival balance is an important feature to maintain tissue homeostasis. Dependence receptors are able to induce either survival or cell death in presence or absence of their ligand, respectively. However, their precise mechanism of action and their physiological importance are still elusive for most of them including the MET receptor.

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How nuclear proteins diffuse and find their targets remains a key question in the transcription field. Dynamic proteins in the nucleus are classically subdiffusive and undergo anomalous diffusion, yet the underlying physical mechanisms are still debated. In this study, we explore the contribution of interactions to the generation of anomalous diffusion by the means of fluorescence spectroscopy and simulation.

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Ets-1 transcription factor overexpression in breast cancers is associated with invasive features and is associated with a poor prognosis. Beyond its role in driving carcinoma cell invasion, in this study, we wished to determine whether Ets-1 overexpression in cancer cells promotes angiogenesis by creating a paracrine pro-invasive environment for endothelial cells as well. To address this question, we set up different co-culture models of cancer cells with endothelial cells.

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Photodynamic therapy (PDT) has developed into a new clinical and non-invasive treatment for cancer over the past 30 years. By the combination of three non-toxic partners, i.e.

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Most cell physiology events are dictated by the integration of perceived signals and the elaboration by cells of adapted answers via the execution of proper transcriptional programs. In order to ensure an optimal control of these answers, many regulation mechanisms have been selected throughout the evolution, thus allowing to fine-tune transcript expression. The transcriptional pause and its release by P-TEFb (Positive Transcription Elongation Factor) have been evidenced two decades ago.

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Unlabelled: The Sd /Cad antigen reported on glycoconjugates of human tissues has an increasingly recognized wide impact on the physio-pathology of different biological systems. The last step of its biosynthesis relies on the enzymatic activity of the β1,4-N-acetylgalactosaminyltransferase-II (B4GALNT2), which shows the highest expression level in healthy colon. Previous studies reported the occurrence in human colonic cells of two B4GALNT2 protein isoforms that differ in the length of their cytoplasmic tail, the long isoform showing an extended 66-amino acid tail.

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Calcium (Ca) release from the endoplasmic reticulum plays an important role in many cell-fate defining cellular processes. Traditionally, this Ca release was associated with the ER Ca release channels, inositol 1,4,5‑triphosphate receptor (IPR) and ryanodine receptor (RyR). Lately, however, other calcium conductances have been found to be intracellularly localized and to participate in cell fate regulation.

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Biochemical studies have revealed that the RNA Polymerase II (RNAPII) pause release is triggered by phosphorylation of the transcription machinery by the positive transcription elongation factor b (P-TEFb). However, there are no direct report that P-TEFb and RNA polymerase II interact in single living cells and the biophysical mechanisms mediating this association are still unclear. Förster resonance energy transfer (FRET) detects molecular interactions at the subcellular level.

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Copepods are small crustaceans capable to survive in various aquatic environments. Their responses to changes in different external factors such as salinity and temperature can be observed at different integration levels from copepod genes to copepod communities. Until now, no thorough observation of the temperature or salinity effect stresses on copepods has been done by optical microscopy.

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We have discovered and developed a series of molecules (thiazole benzenesulfonamides). HA15, the lead compound of this series, displayed anti-cancerous activity on all melanoma cells tested, including cells isolated from patients and cells that developed resistance to BRAF inhibitors. Our molecule displayed activity against other liquid and solid tumors.

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Particle-based photodynamic therapy (PPDT) holds great promise in theranostic applications. Herein, we demonstrate that PPDT based on gold nanorods coated with an indocyanine green (ICG)-loaded silica shell allows for the inactivation of the Crohn's disease-associated adherent-invasive Escherichia coli strain LF82 (E. coli LF82) under pulsed laser light irradiation at 810 nm.

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Article Synopsis
  • TRPM8 is a special protein that has been studied for its potential to help treat or predict prostate cancer.
  • Different versions (isoforms) of TRPM8 act differently in cancer, so it's important to understand what each one does.
  • Research shows that a smaller version called sM8 might help control cancer cell death, so targeting it could be a new way to treat prostate cancer.
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Uncoupling of ERK1/2 phosphorylation from subcellular localization is essential towards the understanding of molecular mechanisms that control ERK1/2-mediated cell-fate decision. ERK1/2 non-catalytic functions and discoveries of new specific anchors responsible of the subcellular compartmentalization of ERK1/2 signaling pathway have been proposed as regulation mechanisms for which dynamic monitoring of ERK1/2 localization is necessary. However, studying the spatiotemporal features of ERK2, for instance, in different cellular processes in living cells and tissues requires a tool that can faithfully report on its subcellular distribution.

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The potential of gold nanorods post-coated with a 20 nm silica shell loaded with verteporfin (Au NRs@SiO2-VP) as efficient near-infrared nanostructures for photodynamic therapy under continuous wave and pulsed-mode excitation to eradicate a virulent strain of E. coli associated with urinary tract infection is described.

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Biofilm formation of Pseudomonas aeruginosa and Staphylococcus aureus on food-contact-surfaces represents a significant risk for the public health. In this context, the present study investigates the relationship between the environmental conditions of biofilm formation and the resistance to disinfectants. Therefore, a static biofilm reactor, called NEC-Biofilm System, was established in order to study the effect of growth temperature (20, 30 and 37°C), and of the surface type (stainless steel and polycarbonate), on biofilm resistance to disinfectants.

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Pseudodiaptomus marinus copepods are small crustaceans living in estuarine areas endowed with exceptional swimming and adaptative performances. Since the external cuticle acts as an impermeable barrier for most dyes and molecular tools for labeling copepod proteins with fluorescent tags are not available, imaging cellular organelles in these organisms requires label free microscopy. Complementary nonlinear microscopy techniques have been used to investigate the structure and the response of their myofibrils to abrupt changes of temperature or/and salinity.

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Article Synopsis
  • Developing alternatives to antibiotics for treating infections caused by Gram-negative uropathogenic E. coli is challenging, necessitating new treatment strategies.
  • The study presents pegylated reduced graphene oxide nanoparticles (rGO-PEG) and gold nanorods coated with rGO-PEG (rGO-PEG-Au NRs) that can effectively kill uropathogenic E. coli UTI89 using a photothermal method, achieving up to 99% bacterial death in just 10 minutes.
  • The rGO-PEG-Au NRs outperformed rGO-PEG in effectiveness, especially when functionalized with specific probes, suggesting a promising new approach for treating urinary infections in a biocompatible manner.
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