Publications by authors named "Laurens Deurhof"
Chromosome substitution lines (CSLs) are tentatively supreme resources to investigate non-allelic genetic interactions. However, the difficulty of generating such lines in most species largely yielded imperfect CSL panels, prohibiting a systematic dissection of epistasis. Here, we present the development and use of a unique and complete panel of CSLs in Arabidopsis thaliana, allowing the full factorial analysis of epistatic interactions.
View Article and Find Full Text PDFNematode migration, feeding site formation, withdrawal of plant assimilates, and activation of plant defence responses have a significant impact on plant growth and development. Plants display intraspecific variation in tolerance limits for root-feeding nematodes. Although disease tolerance has been recognized as a distinct trait in biotic interactions of mainly crops, we lack mechanistic insights.
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- Researchers conducted a VIGS screen using tobacco rattle virus on Nicotiana benthamiana to identify host factors linked to the susceptibility of the tomato spotted wilt orthotospovirus (TSWV).
- The methodology was refined to enable systematic testing of thousands of clones while mitigating the impact of dual viral infections.
- Five clones showed significant resistance to TSWV, with one clone targeting the ribosomal protein S6 gene family, which plays a key role in TSWV susceptibility and is also involved in the replication of various plant RNA viruses.
Article Synopsis
- The tripartite genome of TSWV is combined with two crucial viral proteins to form infectious ribonucleoprotein complexes (RNPs) necessary for viral replication and transcription.
- Analysis of RNPs from TSWV-infected plants showed they contain plant proteins linked to sugar transport and stress response, while those from yeast emphasized RNA processing and ribosome assembly.
- Gene silencing experiments in plants identified four host factors essential for the systemic spread of TSWV and the development of disease symptoms.
Vaccines pave the way out of the SARS-CoV-2 pandemic. Besides mRNA and adenoviral vector vaccines, effective protein-based vaccines are needed for immunization against current and emerging variants. We have developed a virus-like particle (VLP)-based vaccine using the baculovirus-insect cell expression system, a robust production platform known for its scalability, low cost, and safety.
View Article and Find Full Text PDFHybrid crop varieties are traditionally produced by selecting and crossing parental lines to evaluate hybrid performance. Reverse breeding allows doing the opposite: selecting uncharacterized heterozygotes and generating parental lines from them. With these, the selected heterozygotes can be recreated as F1 hybrids, greatly increasing the number of hybrids that can be screened in breeding programs.
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