Heme oxygenase-1 activity after excitotoxic injury: immunohistochemical localization of bilirubin in neurons and astrocytes and deleterious effects of heme oxygenase inhibition on neuronal survival after kainate treatment.

An increased expression of the inducible form of heme oxygenase (HO), HO-1, is found in the hippocampus after kainate injection, but thus far it is unclear whether the HO-1 is enzymatically active. The present study was carried out, using monoclonal antibodies to bilirubin and HO-1 and histochemical staining for iron, to compare the products of HO enzymatic activity, bilirubin and iron, with HO-1 expression in the kainate-lesioned hippocampus. There was a close correlation between bilirubin and HO-1 expression, and both bilirubin and HO-1 were observed in damaged neurons at early times, and astrocytes at later times (weeks), after kainate injection.

Induction of astrocytic cytoplasmic phospholipase A2 and neuronal death after intracerebroventricular carrageenan injection, and neuroprotective effects of quinacrine.

Glial reaction is often associated with nervous tissue injury, but thus far, few studies have examined whether it can be a cause of neuronal injury. We now study the effect of intracerebroventricular injection of a carrageenan on cytoplasmic phospholipase A(2) (cPLA(2)) expression and neuronal injury in the hippocampus. The enzyme cPLA(2) hydrolyzes neural membrane glycerophospholipids and generates precursors for proinflammatory mediators.