Engineered nanoparticles promote cardiac tropism of AAV vectors.

Cardiac muscle targeting is a notoriously difficult task. Although various nanoparticle (NP) and adeno-associated viral (AAV) strategies with heart tissue tropism have been developed, their performance remains suboptimal. Significant off-target accumulation of i.

Surface Geometry of Cargo-less Gold Nanoparticles Is a Driving Force for Selective Targeting of Activated Neutrophils to Reduce Thrombosis in Antiphospholipid Syndrome.

Antiphospholipid syndrome (APS) is an autoimmune disease characterized by recurrent arterial, venous, and microvascular thrombosis where activated neutrophils play a determinant role. However, neutrophils are challenging to target given their short lifespan in circulation and spontaneous activation. Screening of a small library of gold nanoparticles (AuNPs) led to the discovery of a formulation capable of targeting activated neutrophil attachment and has demonstrated that star-shaped, anti-PSGL-1-antibody-coated AuNPs (aPSGL-1-AuNPs) were more efficacious compared with other shapes of AuNPs.

Local Treatment of Non-small Cell Lung Cancer with a Spray-Dried Bevacizumab Formulation.

Local delivery of biotherapeutics to the lung holds great promise for treatment of lung diseases, but development of physically stable, biologically active dry powder formulations of large molecules for inhalation has remained a challenge. Here, spray drying was used to manufacture a dry powder pulmonary formulation of bevacizumab, a monoclonal antibody approved to treat non-small cell lung cancer (NSCLC) by intravenous infusion. By reformulating bevacizumab for local delivery, reduced side effects, lower doses, and improved patient compliance are possible.

An electron paramagnetic resonance mobile universal surface explorer.

Many samples cannot be studied by electron paramagnetic resonance (EPR) spectroscopy because they are too large to fit into the spectrometer and too precious to be destructively sampled for study. An EPR mobile universal surface explorer (MOUSE), also known as a unilateral EPR spectrometer, was constructed for studying this class of sample. The EPR MOUSE can nondestructively record a low frequency EPR (LFEPR) spectrum of a small region of any size object by placing the MOUSE against the object.

Low Frequency EPR and EMR Point Spectroscopy and Imaging of a Surface.

Low frequency electron paramagnetic resonance (LFEPR) spectrometers operating between 100 and 500 MHz typically have large-volume magnets that accommodate large samples. LFEPR spectroscopy with a 2.9 mm diameter surface coil was used to record point spectra and image the spatial distribution of the spin probe 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and electrophotographic toner in printed letters on a flat surface.