Comparison of Juvenile Feed Protocols on Growth and Spawning in Zebrafish.

Over the past 2 decades, zebrafish, , have become a mainstream laboratory animal model, yet zebrafish husbandry practices remain far from standardized. Feeding protocols play a critical role in the health, wellbeing, and productivity of zebrafish laboratories, yet they vary significantly between facilities. In this study, we compared our current feeding protocol for juvenile zebrafish (30 dpf to 75 dpf), a 3:1mixture of fish flake and freeze-dried krill fed twice per day with live artemia twice per day (FKA), to a diet of Gemma Micro 300 fed once per day with live artemia once per day (GMA).

Haematopoietic stem cell-dependent Notch transcription is mediated by p53 through the Histone chaperone Supt16h.

Haematopoietic stem and progenitor cells (HSPCs) have been the focus of developmental and regenerative studies, yet our understanding of the signalling events regulating their specification remains incomplete. We demonstrate that supt16h, a component of the Facilitates chromatin transcription (FACT) complex, is required for HSPC formation. Zebrafish supt16h mutants express reduced levels of Notch-signalling components, genes essential for HSPC development, due to abrogated transcription.

Body Condition Scoring for Adult Zebrafish ().

Body condition scoring (BCS) is a simple, rapid, noninvasive tool used to assess body condition in animals. In this study, we developed and validated a diagram-based BCS for adult zebrafish (), a popular research model. After receiving 20 min of hands-on training regarding the scoring system, 5 people each rated 95 adult zebrafish.