Comparative Evaluation of the Foot-and-Mouth Disease Virus Permissive LF-BK αβ Cell Line for Senecavirus A Research.

Senecavirus A (SVA) is a member of the family and enzootic in domestic swine. SVA can induce vesicular lesions that are clinically indistinguishable from Foot-and-mouth disease, a major cause of global trade barriers and agricultural productivity losses worldwide. The LF-BK αβ cell line is a porcine-derived cell line transformed to stably express an αβ bovine integrin and primarily used for enhanced propagation of Foot-and-mouth disease virus (FMDV).

Detection of African swine fever virus utilizing the portable MatMaCorp ASF detection system.

African swine fever (ASF) has emerged as a major threat to domestic and wild suid populations, and its continued spread threatens commercial swine production worldwide. The causative agent of ASF, African swine fever virus (ASFV), possesses a linear, double stranded DNA genome. Traditional detection of ASFV relies on laboratory-based virus isolation or real-time PCR of samples, typically blood or spleen, obtained from suspect cases.

Generation and characterization of genetically stable heterohybridomas producing foot-and-mouth disease virus-specific porcine monoclonal antibodies.

This report covers the methodology for generation of stable heterohybridoma clones producing Foot-and-mouth disease virus (FMDV) reactive porcine monoclonal antibodies (mAbs). Swine received five inoculations of an inactivated O1 Manisa FMDV vaccine prior to the harvest of splenocytes. Due to the lack of a species-specific hybridoma fusion partner, the Sp2/0 murine myeloma cell line was utilized for the formation of porcine-murine heterohybridoma clones.