Creation of an Acellular Vaginal Matrix for Potential Vaginal Augmentation and Cloacal Repair.

Study Objective: Our aim was to use porcine vagina to create a vaginal matrix and test its cellular biocompatibility.

Design, Setting, And Participants: Vagina was harvested from pigs and decellularized (DC) using a combination of detergents (Triton X-100 and sodium deoxycholate) and enzymes (DNAse/RNAse).

Interventions: The presence of cellular material, collagen structural integrity, and basement membrane proteins were assessed histologically.