EASI Transformation Protocol: An Agrobacterium-Mediated Transient Transformation Protocol for Catharanthus roseus Seedlings.

Catharanthus roseus produces medicinal terpenoid indole alkaloids, including the critical anti-cancer compounds vinblastine and vincristine in its leaves. Recently, we developed a highly efficient transient expression method relying on Agrobacterium-mediated transformation of seedlings to facilitate rapid and high-throughput studies on the regulation of terpenoid indole alkaloid biosynthesis in C. roseus .

The regulation of , a transcriptional repressor of monoterpenoid indole alkaloid biosynthetic genes in .

Cys/His-type (CH) zinc finger proteins, such as ZCT1, are an important class of transcription factors involved in growth, development, and stress responses in plants. In the medicinal plant the zinc finger transcription factor (ZCT) family represses monoterpenoid indole alkaloid (MIA) biosynthetic gene expression Here, we report the analysis of the promoter, which contains several hormone-responsive elements. is responsive to not only jasmonate, as was previously known, but is also induced by the synthetic auxin, 1-naphthalene acetic acid (1-NAA).

EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function in Seedlings.

The plant is the exclusive source of the valuable anticancer terpenoid indole alkaloids, vinblastine (VB) and vincristine (VC). The recent availability of transcriptome and genome resources for necessitates a fast and reliable method for studying gene function. In this study, we developed an -mediated transient expression method to enable the functional study of genes rapidly , conserving the compartmentalization observed in the VB and VC pathway.