Publications by authors named "Lauren B Woods"
Article Synopsis
- - Preclinical studies indicate that off-target effects from RNA interference contribute significantly to hepatotoxicity in GalNAc-siRNA conjugates.
- - Researchers developed a new strategy called enhanced stabilization chemistry plus (ESC+) which effectively reduces off-target binding while preserving the intended on-target effects in RNA therapies.
- - This approach was successfully applied to ALN-HBV, leading to a redesigned version (ALN-HBV02) with better specificity and safety, allowing it to resume clinical development.
siRNAs containing 2'-fluorinated Northern-methanocarbacyclic (2'-F-NMC) nucleotides: in vitro and in vivo RNAi activity and inability of mitochondrial polymerases to incorporate 2'-F-NMC NTPs.
Masaaki Akabane-Nakata Namrata D Erande Pawan Kumar Rohan Degaonkar Jason A Gilbert Lauren Blair Woods
Nucleic Acids Res
March 2021
We recently reported the synthesis of 2'-fluorinated Northern-methanocarbacyclic (2'-F-NMC) nucleotides, which are based on a bicyclo[3.1.0]hexane scaffold.
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- Various chemical modifications boost the effectiveness of small interfering RNAs (siRNAs) while minimizing unwanted side effects, such as immune reactions and toxicity from byproducts.* -
- The study focuses on synthesizing and evaluating two specific forms of C-methyl guanosine (the (R) and (S) isomers) in siRNA, showing that they can protect against different types of enzyme degradation.* -
- Structural analyses reveal that while the (R) isomer has lower thermal stability compared to the (S) isomer, both modifications maintain the effectiveness of siRNA and have minimal effects on mitochondrial DNA polymerase activity.*
Changes in the multi-level physical structure of biological features going from cellular to tissue level composition is a key factor in many major diseases. However, we are only beginning to understand the role of these structural changes because there are few dedicated multiscale imaging platforms with sensitivity at both the cellular and macrostructural spatial scale. A single platform reduces bias and complications from multiple sample preparation methods and can ease image registration.
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