Activation of a caspase-3-independent mode of cell death associated with lysosomal destabilization in cultured human retinal pigment epithelial cells (ARPE-19) exposed to 7beta-hydroxycholesterol.

Purpose: To characterize the possible cytotoxic effects of oxysterols (7beta-hydroxycholesterol (7beta-OH), 25-hydroxycholesterol (25-OH)) in human retinal pigment epithelial cells (ARPE-19) and to detail the relationships between some of these effects.

Methods: ARPE-19 cells were treated with 7beta-OH and 25-OH. Cell viability was measured with the MTT assay.

Comparison of face-down and seated position after idiopathic macular hole surgery: a randomized clinical trial.

Purpose: To compare two therapeutic modalities on anatomic and functional results after idiopathic macular hole (MH) surgery: seated vs face-down position.

Design: Multicenter, prospective, randomized trial.

Setting: University Hospital Dijon and University Hospital Nancy.

Incidence of retinal detachment after macular surgery: a retrospective study of 634 cases.

Aims: To evaluate the rate of retinal detachment after macular surgery.

Methods: A retrospective non-randomised study of 634 macular surgery procedures was undertaken in two academic centres. Idiopathic macular hole (IMH) surgery (n = 272) and epiretinal membrane (ERM) surgery (n = 362) were performed between 2000 and 2003.

Oxysterols induced inflammation and oxidation in primary porcine retinal pigment epithelial cells.

Purpose: Aging is associated with an accumulation of cholesterol esters in the Bruch membrane. Cholesterol esters are prone to undergo oxidation and generate oxysterols that have cytotoxic and proinflammatory properties. We investigated the effects of three oxysterols on mitochondrial dysfunctions, inflammation, and oxidative stress in primary cultures of porcine retinal pigment epithelial (RPE) cells.