Publications by authors named "Laure Magnan"
Cell-assembled extracellular matrix (CAM) has been used to produce vascular grafts. While these completely biological vascular grafts performed well in clinical trials, the in vivo remodeling and inflammatory response of this truly "bio" material has not yet been investigated. In this study, human CAM yarns were implanted subcutaneously in nude rats to investigate the innate immune response to this matrix.
View Article and Find Full Text PDFWe have created entirely biological tissue-engineered vascular grafts (TEVGs) using sheets of cell-assembled extracellular matrix (CAM) produced by human fibroblasts . A large animal TEVG would allow long-term pre-clinical studies in a clinically relevant setting (graft size and allogeneic setting). Therefore, canine, porcine, ovine, and human skin fibroblasts were compared for their ability to form CAM sheets.
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- In tissue engineering, the extracellular matrix produced by cells serves as a scaffold that offers structural support, and researchers have developed a method to mass-produce Cell-Assembled extracellular Matrix (CAM) using human fibroblasts.
- This article introduces CAM yarns that exhibit diverse physical and mechanical properties, usable from simple sutures to creating fully biological tissue-engineered vascular grafts (TEVGs) with high mechanical strength for implantation.
- The innovative technique combines biological materials with textile assembly methods to create versatile medical textiles, which meet or exceed clinical standards for strength and integration into the human body.
Cartography of the mechanical properties of the human amniotic membrane.
J Mech Behav Biomed Mater
November 2019
Because of its low immunogenicity, biological properties, and high availability, the Human Amniotic Membrane (HAM) is widely used in the clinic and in tissue engineering research. However, while its biological characteristics are well described, its mechanical properties remain understudied especially in terms of inter- and intra-HAM variability. To guide bioengineers in the use of this natural biomaterial, a detailed cartography of the HAM's mechanical properties was performed.
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- The study investigated the Cell-Assembled extracellular Matrix (CAM) created from human skin fibroblasts, confirming its effectiveness as a biological vascular graft previously tested in clinical settings.
- Researchers analyzed the composition and organization of CAM, identifying over 50 proteins and highlighting its dense collagen network and tissue-like properties.
- The findings showed that a devitalization process (dehydration) used for storage did not negatively impact the mechanical strength or structural organization of CAM, making it suitable for off-the-shelf use without adverse effects on its functionality.
Article Synopsis
- - The regulation of RhoGTPases like RhoA is crucial for cell migration, with p190RhoGAP (p190A) acting as the primary negative regulator by localizing to cell membrane protrusions and activating RhoA's GTPase activity.
- - Researchers identified a specific domain in p190A, called the protrusion localization sequence (PLS), that is essential for its targeting to leading edges and is also necessary for its negative regulatory function.
- - The study found that cortactin, an F-actin binding protein, interacts with the PLS to assist in p190A localization, and mutations in the PLS linked to cancer can disrupt p190A localization and its effectiveness in