Subretinal Therapy: Technological Solutions to Surgical and Immunological Challenges.

Recent advances in ocular gene and cellular therapy rely on precisely controlled subretinal delivery. Due to its inherent limitations, manual delivery can lead to iatrogenic damage to the retina, the retinal pigment epithelium, favor reflux into the vitreous cavity. In addition, it suffers from lack of standardization, variability in delivery and the need to maintain proficiency.

Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach.

Background: Blood-retinal barrier cells are known to exhibit a massive phenotypic change during experimental autoimmune uveitis (EAU) development. In an attempt to investigate the mechanisms of blood-retinal barrier (BRB) breakdown at a global level, we studied the gene regulation of total retinal cells and retinal endothelial cells during non-infectious uveitis.

Methods: Retinal endothelial cells were isolated by flow cytometry either in Tie2-GFP mice (CD31 CD45 GFP cells), or in wild type C57BL/6 mice (CD31 CD45 endoglin cells).

MHC class II expression and potential antigen-presenting cells in the retina during experimental autoimmune uveitis.

Background: Controversy exists regarding which cell types are responsible for autoantigen presentation in the retina during experimental autoimmune uveitis (EAU) development. In this study, we aimed to identify and characterize the retinal resident and infiltrating cells susceptible to express major histocompatibility complex (MHC) class II during EAU.

Methods: EAU was induced in C57BL/6 mice by adoptive transfer of autoreactive lymphocytes from IRBP1-20-immunized animals.

Intraocular T-cell Lymphoma: Clinical Presentation, Diagnosis, Treatment, and Outcome.

Purpose: To report on the clinical data of seven patients with T-cell intraocular lymphoma (IOL).

Methods: Retrospective case series.

Results: Seven immunocompetent patients, 12 eyes, 6 women, with T-cell-IOL were included from five countries.

Comparison of rubella virus- and herpes virus-associated anterior uveitis: clinical manifestations and visual prognosis.

Purpose: To compare the clinical characteristics and visual prognosis of patients with anterior uveitis (AU) and intraocular fluid analysis positive for rubella virus (RV), herpes simplex virus (HSV), or varicella zoster virus (VZV).

Design: Retrospective, observational study.

Participants: The study included 106 patients with AU and positive polymerase chain reaction (PCR) results, Goldmann-Witmer coefficients (GWCs), or both, for RV (n = 57), HSV (n = 39), or VZV (n = 10).

Rifabutin-associated panuveitis with retinal vasculitis in pulmonary tuberculosis.

Introduction: Rifabutin-associated uveitis has been reported frequently in AIDS patients and more rarely in immunocompetent patients. It is characterized clinically by anterior acute uveitis. Only a few poorly documented cases of rifabutin-induced panuveitis with retinal vasculitis have been reported.