Publications by authors named "Laura Zelenka"
Article Synopsis
- The study focuses on the communication between microglia and astrocytes, the main immune cells in the central nervous system, emphasizing their role in brain health and disease.
- Researchers examined crosstalk between genetically modified (TLR2-KO) and normal (wild-type) microglia and astrocytes, discovering significant TNF release by TLR2-KO astrocytes when exposed to wild-type microglial signals.
- The study utilized RNA sequencing to identify genes involved in this interaction and affirmed findings through co-culturing, underscoring the need for better methods to isolate glial cells for future research.
Background: The crosstalk and reactivity of the cell type glia, especially microglia and astrocytes, have progressively gathered research attention in understanding proper brain function regulated by the innate immune response. Therefore, methods to isolate highly viable and pure glia for the analysis on a cell-specific level are indispensable.
New Method: We modified previously established techniques: Animal numbers were reduced by multiple microglial harvests from the same mixed glial culture, thereby maximizing microglial yields following the principles of the 3Rs (replacement, reduction, and refinement).