Overexpression of in Affects Lignin Biosynthesis Without Altering Redox Homeostasis.

Class III plant peroxidases (Prxs) are involved in the oxidative polymerization of lignins. Z Jacq. Basic peroxidase (ZePrx) has been previously characterized as capable of catalyzing this reaction and the role in lignin biosynthesis of several of its homologous has been previously confirmed.

Genetic transformation of grapevine cells using the minimal cassette technology: the need of 3'-end protection.

The use of minimal cassettes (linear DNA comprising promoter+open reading frame+terminator) for genetic transformation offers a means of transforming plant cells by biolistics without introducing unwanted sequences. However, a species-specific approach of the factors involved in successful transformation with this technology is advisable. Protection of the minimal cassette upstream promoter and downstream terminator may be necessary due to the nuclease activity of target plant material.