Preparation of a three-dimensional extracellular matrix by decellularization of rabbit livers.

Introduction: the availability of transplantable livers is not sufficient to fulfill the current demand for grafts, with the search for therapeutic alternatives having generated different lines of research, one of which is the use of decellularized three-dimensional biological matrices and subsequent cell seeding to obtain a functional organ.

Objective: to produce a decellularization protocol from rabbit liver to generate a three-dimensional matrix.

Methods: a combination of physical, chemical (Triton X-100 and SDS) and enzymatic agents to decellularize rabbit livers was used.