Publications by authors named "Laura Okmane"
H, C and N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium.
Biomol NMR Assign
December 2024
A glycoside hydrolase family 45 (GH45) enzyme from the white-rot basidiomycete fungus Phanerochaete chrysosporium (PcCel45A) was expressed in Pichia pastoris with C and N labelling. A nearly complete assignment of H, C and N backbone resonances was obtained, as well as the secondary structure prediction based on the assigned chemical shifts using the TALOS-N software. The predicted secondary structure was almost identical to previously published crystal structures of the same enzyme, except for differences in the termini of the sequence.
View Article and Find Full Text PDFHere we describe the first crystal structure of a beta-1,4-endoglucanase from a brown-rot fungus, Gloeophyllum trabeum GtCel45A, which belongs to subfamily C of glycoside hydrolase family 45 (GH45). GtCel45A is ~ 18 kDa in size and the crystal structure contains 179 amino acids. The structure is refined at 1.
View Article and Find Full Text PDFArticle Synopsis
- The study compares the enzymatic activity of a specific β-1,4-D-endoglucanase (MeCel45A) from blue mussels with other enzymes in the glycoside hydrolase family 45, focusing on their efficiency in hydrolyzing barley beta-glucan, konjac glucomannan, and carboxymethyl cellulose.
- The crystal structure of MeCel45A is documented, revealing its unique features and performance, especially in colder temperatures where it retains high activity levels.
- While all enzymes exhibit inverting glycoside hydrolase activity, MeCel45A and the C subfamily enzymes show mannanase activity, while subfamily A yielded glucose, showcasing distinct functionalities among these