Publications by authors named "Laura N Quick"
Low fluid shear force, including that encountered in microgravity models, induces bacterial responses, but the range of bacteria capable of responding to this signal remains poorly characterized. We systematically analyzed a range of Gram negative Enterobacteriaceae for conservation of the low-shear modeled microgravity (LSMMG) response using phenotypic assays, qPCR, and targeted mutations. Our results indicate LSMMG response conservation across Enterobacteriacae with potential variance in up- or down-regulation of a given response depending on genus.
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- The study builds on previous research by demonstrating that cloned SPI-1 from Salmonella Typhimurium creates an adherent biofilm and cell clumps due to high expression of type III secretion functions.
- This biofilm formation is characterized by the secretion of SPI-1 protein substrates and occurs regardless of the presence of known biofilm-related genes, suggesting a unique mechanism of aggregation.
- The findings have implications for potential applications in creating engineered biofilms, enhancing protein purification techniques, and improving antigen presentation.
Self-transmissible IncP R995 plasmids with alternative markers and utility for Flp/FRT cloning strategies.
J Microbiol Biotechnol
November 2011
The IncP plasmid R995 has been a useful self-transmissible, broad-host-range vector for a number of applications including the recombinase/conjugation-based cloning of large genomic DNA segments. However, R995 derivatives (or related plasmids) expressing a wide range of different resistance markers and Flp recombinase target sites do not exist in the literature. In addition, documented strategies for applying such plasmids in cloning applications that take advantage of conjugation for the convenient isolation and recovery of constructs are extremely limited.
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- Salmonella enterica serovar Typhimurium has a set of genes, including ydcI, that help it adapt to low fluid shear force conditions, which boost its virulence and affect other traits.
- The ydcI gene encodes a conserved DNA binding protein that also plays a role in its own regulation, and is part of a wider stress resistance system in the bacteria.
- Mutations in ydcI lead to increased sensitivity to acid stress and improved invasion of host cells when ydcI expression is induced, suggesting its relevance in both stress response and virulence factors.
A series of vectors with alternative antibiotic resistance markers for use in lambda Red recombination.
J Microbiol Biotechnol
April 2010
A target bacterial strain of interest for use in Red-based recombineering may already encode resistance to antibiotic markers used with current Red recombination tools such that the resistance cannot be removed. Such cases include those where markers are needed to maintain an unstable genetic element co-resident in the strain or those where the genetic source of resistance is not known. We report the availability of PCR templates with FRT-flanked mutagenesis cassettes and plasmids encoding Red recombination functions that contain marker combinations not currently available on widely disseminated lambda Red molecular reagents.
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