Introduction: Fungal mitogenomes exhibit remarkable variation in conformation, size, gene content, arrangement and expression, including their intergenic spacers and introns.
Methods: The complete mitochondrial genome sequence of the mycoparasitic fungus was determined using the Illumina next-generation sequencing technology. We used data from our recent Illumina NGS-based project of genome sequencing to study its mitochondrial genome.
The agricultural industries generate lignocellulosic wastes that can be modified by fungi to generate high value-added products. This work aimed to analyze the efficiency and the cost-effectiveness of the bioconversion of sugarcane and cassava bagasses using low-cost homemade enzymatic cocktails from LBM 134. Both bagasses were pretreated with a soft alkaline solution without any loss of polysaccharides.
View Article and Find Full Text PDFAspergillus is a genus of filamentous and cosmopolitan fungi that includes important species for medical mycology, food, basic research and agro-industry areas. section are efficient producers of hydrolytic enzymes such as cellulases that are employed in the cellulose conversion. Hence, the search of new cellulolytic isolates and their correct identification is important for carrying out safe biotechnological processes.
View Article and Find Full Text PDFThe aim of this study was to investigate the bioremediation potential of polychlorinated biphenyls (PCBs) in soil, mimicking three strategies: (a) mycoaugmentation: by the addition of Trametes sanguinea and Pleurotus sajor-caju co-cultures immobilized on sugarcane bagasse; (b) biostimulation: by supplementation of sugarcane bagasse; and (c) natural attenuation: no amendments. The experiments were done in microcosms using Ultisol soil. Remediation effectiveness was assessed based on pollutants content, soil characteristics, and ecotoxicological tests.
View Article and Find Full Text PDFCurrently, agroindustrial wastes are little used for generating value-added products; hence, their use of these waste to produce enzymatic cocktails for the conversion of lignocellulosic biomass to fermentable sugars is a very interesting alternative in the second-generation bioethanol process. The Ascomycota fungus LBM 134 produces hydrolytic enzymes in large proportions. In this work, LBM 134 was grown on sugarcane and cassava bagasses under optimized conditions.
View Article and Find Full Text PDFIn this study, we evaluated the presence of lipases in twenty fungal strains of the genus using an efficient and low-cost method with a view to an application in the treatment of cooking oil residues. The Paranaense rainforest is one of the most biodiverse places on the planet, making it the most likely site to find new fungal strains with lipolytic potential. The objective of this study was to determine the lipolytic potential and the isoenzyme profile of fungi belonging to the genus isolated from the Paranaense rainforest.
View Article and Find Full Text PDFAgricultural practices generate lignocellulosic waste that can be bioconverted by fungi to generate value-added products such as biofuels. In this context, fungal enzymes are presented as an alternative for their use in the hydrolysis of cellulose to sugars that can be fermented to ethanol. The aim of this work was to characterize LBM 033 strain and to analyze its efficiency in the hydrolysis of cellulosic substrates, including barley straw.
View Article and Find Full Text PDFSt. Hil (yerba mate) is an important crop in the north of Argentina, mainly in Misiones province. The application of as a biocontroller and biofertilizer can replace or reduce the use of agrochemicals, decreasing the negative ecological impact.
View Article and Find Full Text PDFThe aim of the present study was to assess the ability of different white-rot fungi to tolerate polychlorinated biphenyls (PCBs) using predictive mycology, by relating fungal growth inhibition to ligninolityc enzyme secretion. Fungal strains were grown in the presence of PCBs in solid media and their radial growth values were modelled through the Dantigny-logistic like function in order to estimate the time required by the fungal colonies to attain half their maximum diameter. The principal component analysis (PCA) revealed an inverse correlation between strain tolerance to PCBs and the laccase secretion over time, being laccase production closely associated with fungal growth capacity.
View Article and Find Full Text PDFstrain POS7 produces significantly large amounts of cellulase enzymes in solid-state fermentation. The Illumina-based sequence analysis reveals an approximate genome size of 36.6 Mbp, with a G+C content of 48.
View Article and Find Full Text PDFOxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp.
View Article and Find Full Text PDFNine aromatic compounds (caffeic acid, syringaldehyde, vanillic acid, guaiacol, vanillin, sinapic acid, syringol, syringic acid and ferulic acid) and four metallic compounds (CuSO4, AgNO3, MnSO4, and CaCl2) were tested for their ability to increase laccase (Lac) activity in the ligninolytic basidiomycete Phlebia brevispora BAFC 633. The addition of syringaldehyde, syringol, guaiacol, sinapic acid, vanillin, ferulic acid and CuSO4 showed a positive effect on fungal growth; however, it decreased dramatically with the addition of AgNO3 and did not undergo changes in the presence of CaCl2 or MnSO4. Lac activity increased with the addition of all the compounds tested, depending on the concentration and the day of culture.
View Article and Find Full Text PDFWhite rot fungi have an enzymatic system producing oxidative and hydrolytic enzymes that act on the degradation of certain components of the cell wall. They can be applied in several technological processes, such as paper industry, bio-fuels and environmental pollution. Laccases are multi-copper enzymes of wide substrate specificity and high non-specific oxidation capacity that use molecular oxygen to oxidize various aromatic compounds, and are highly relevant biotechnological applications.
View Article and Find Full Text PDFFungi may be selected as models for gene expression studies and further adaptation for biotechnological enzyme production. The aim of this work was to evaluate laccase production and to analyze the effect of Cu(2+) on selected fungi natives of Misiones, Ganoderma applanatum (strain F), Peniophora sp. (BAFC 633), Pycnoporus sanguineus (BAFC 2126) and Coriolus versicolor f.
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