Mapping QTLs for blight resistance and morpho-phenological traits in inter-species hybrid families of chestnut ( spp.).

Chestnut blight (caused by ), together with root rot (caused by ), has nearly extirpated American chestnut () from its native range. In contrast to the susceptibility of American chestnut, many Chinese chestnut () genotypes are resistant to blight. In this research, we performed a series of genome-wide association studies for blight resistance originating from three unrelated Chinese chestnut trees (Mahogany, Nanking and M16) and a Quantitative Trait Locus (QTL) study on a Mahogany-derived inter-species F2 family.

Defining the 'HoneySweet' insertion event utilizing NextGen sequencing and a de novo genome assembly of plum (Prunus domestica).

'HoneySweet' plum (Prunus domestica) is resistant to Plum pox potyvirus, through an RNAi-triggered mechanism. Determining the precise nature of the transgene insertion event has been complicated due to the hexaploid genome of plum. DNA blots previously indicated an unintended hairpin arrangement of the Plum pox potyvirus coat protein gene as well as a multicopy insertion event.

Dissecting Resistance to in Interspecific Hybrid Chestnut Crosses Using Sequence-Based Genotyping and QTL Mapping.

The soilborne oomycete -which causes root rot, trunk cankers, and stem lesions on an estimated 5,000 plant species worldwide-is a lethal pathogen of American chestnut () as well as many other woody plant species. is particularly damaging to chestnut and chinquapin trees ( spp.) in the southern portion of its native range in the United States due to relatively mild climatic conditions that are conductive to disease development.

Comparison of peach and Arabidopsis genomic sequences: fragmentary conservation of gene neighborhoods.

We examined the degree of conservation of gene order in two plant species, Prunus persica (peach) and Arabidopsis thaliana (thale cress), whose lineages diverged more than 90 million years ago. In the three peach genomic regions studied, segments with a gene order congruent with A. thaliana were short (two to three genes in length); and for any peach region, corresponding segments were found in diverse locations in the A.

High-throughput targeted SSR marker development in peach (Prunus persica).

Simple sequence repeats (SSRs) have proven to be highly polymorphic, easily reproducible, codominant markers. However, developing an SSR map is very time consuming and expensive, and most SSRs are not specifically linked to gene loci of immediate interest. The ideal situation would be to combine a high-throughput, relatively inexpensive mapping technique with rapid identification of SSR loci in mapped regions of interest.