Publications by authors named "Laura Krivko"

Wastewater-based epidemiology (WBE) is a rapid and cost-effective method that can detect SARS-CoV-2 genomic components in wastewater and can provide an early warning for possible COVID-19 outbreaks up to one or two weeks in advance. However, the quantitative relationship between the intensity of the epidemic and the possible progression of the pandemic is still unclear, necessitating further research. This study investigates the use of WBE to rapidly monitor the SARS-CoV-2 virus from five municipal wastewater treatment plants in Latvia and forecast cumulative COVID-19 cases two weeks in advance.

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In 2020, ASF occurred in wild boars throughout Latvia and Lithuania, and more than 21,500 animals were hunted and tested for the presence of the virus genome and antibodies in the framework of routine disease surveillance. The aim of our study was to re-examine hunted wild boars that tested positive for the antibodies and negative for the virus genome in the blood (n = 244) and to see if the virus genome can still be found in the bone marrow, as an indicator of virus persistence in the animal. Via this approach, we intended to answer the question of whether seropositive animals play a role in the spread of the disease.

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Article Synopsis
  • African swine fever (ASF) is a serious viral disease impacting pigs globally, with significant outbreaks occurring in Europe since 2014, affecting 14 countries, including Italy and North Macedonia in 2022.
  • To understand the ASF virus's spread, researchers sequenced 382 virus isolates from 2007 to 2022, using targeted PCR techniques to analyze specific genetic regions.
  • The results revealed 24 distinct ASF virus groups in Europe and identified unique mutations linking recent outbreaks in North Macedonia to neighboring countries, providing insights for improved genotyping and monitoring of the disease.
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