Generation of sheep with defined FecB and TBXT mutations and porcine blastocysts with KCNJ5 mutation using prime editing.

Background: Rewriting the genomes of living organisms has been a long-standing aim in the biological sciences. The revelation of the CRISPR/Cas9 technology has revolutionized the entire biological field. Since its emergence, this technology has been widely applied to induce gene knockouts, insertions, deletions, and base substitutions.